可直接在猪血清中准确检测猪瘟病毒E2抗体的免校准便携式磁弹性装置

Calibrating-free Portable Magnetoelastic Device for Accurate Detection of Anti-CSFV-E2 in Porcine Serum

【目的】设备制造之间的误差校准已成为各种检测方法实际应用的难题。【方法】提出一种便携式磁弹性(ME)生物检测装置,用于实现免校准检测猪瘟病毒E2抗体(anti-CSFV E2),基于磁致伸缩效应,通过监测anti-CSFV E2引起的共振频率偏移反应待测物浓度。该装置由两部分组成,分别为磁弹性试纸和智能磁弹性共振频率检测仪,试纸体积非常小,外形尺寸约为ELISA试剂盒大小的1/20.使用ELISA试剂盒作为对照,通过检测相同浓度的猪血清样本来验证便携式ME装置的准确性。【结果】ELISA试剂盒与免校准便携式ME装置的检测结果匹配良好,准确率为98%.采用便携式ME装置对不同浓度的anti-CSFV E2进行检测,实验结果表明灵敏度为7.20 Hz/(μg/mL),线性范围为0.1μg/mL~100μg/mL,该装置无需校准即可快速、准确地检测anti-CSFV E2,具有良好的特异性和稳定性。【结论】免校准便携式ME装置为猪瘟诊断和抗猪瘟疫苗接种效果的监测提供了一种有效方法。

x【Purposes】Error calibration between equipment manufacturing has become a prac-tical challenge for various inspection methods.【Methods】In this paper,a portable magnetoelastic(ME)biodetection device is proposed for calibration-free detection of classical swine fever vi-rus E2 antibody(anti-CSFV E2),on the basis of magnetostrictive effect,by monitoring the reso-nance frequency shift caused by anti-CSFV E2 to react to the concentration of analytes.The de-vice consists of two parts,namely the magnetoelastic test strip and the intelligent magnetoelastic resonance frequency detector.The test strip is very small,and the external dimension is about 1/20 of the size of the ELISA kit.By using the ELISA kit as a control,the accuracy of the porta-ble ME device is verified by detecting the same concentration of pig serum samples.【Findings】The results show that the detection results of the calibration-free portable ME device and the ELISA kit have good consistency,and the accuracy rate is 98%.The experimental results show that the sensitivity is 7.20 Hz/(μg/mL)and the linear range is 0.1~100μg/mL,and the device can quickly and accurately detect anti-CSFV E2 without calibration,with good specificity and sta-bility.【Conclusions】The calibration-free portable ME unit provides an effective method for the diagnosis of swine fever and the monitoring of the effectiveness of anti-swine fever vaccination.