樱桃砧木抗盐性筛选及转录组测序分析

Screening of Salt Tolerance and Transcriptome Sequencing of Cherry Rootstocks

樱桃对盐碱敏感,筛选耐盐碱砧木意义重大。本研究以4种樱桃砧木‘考特’‘马哈利’‘ZY-1’‘兰丁2号’为试验材料,用0.1 mol/L Na Cl溶液浇灌,测定高盐条件下樱桃抗氧化酶和光合指标,筛选抗性砧木,同时对其转录组进行分析。结果表明,以‘ZY-1’为砧木的嫁接苗在高盐胁迫条件下H2O2产生量最低,保护性酶CAT、POD和SOD活性最高,而且其净光合速率降幅最小,故相较其他材料‘ZY-1’砧木具有较强的抗盐性。同时对高盐胁迫下以‘ZY-1’为砧木的嫁接苗进行转录组测序,获得51.52 Gb的Clean reads,各测序样品的Q20值均大于97.66%,GC含量为41.39%,其测序结果可行性好;通过数据库比对,共有38 861条Clean data得到基因功能注释,其中包括盐胁迫24和48 h时差异表达基因763和2 065个。差异表达基因的生物学功能分析表明,参与激素信号转导、淀粉和蔗糖代谢、转录因子、抗氧化酶、光合及离子转运等的基因可能在樱桃抗盐过程中发挥重要作用。

Cherry(Prunus avium L.)is sensitive to salt and alkali,so it is of great significance to screen salt tolerant rootstocks.In thisstudy,four cherryrootstocks,‘Colt',‘Mahaleb',‘ZY-1'and‘Landing2'were irrigated with 0.1 mol/L NaCl solution to determine the antioxidant enzymes and photosynthetic indexes,screen resistant rootstocks and analyze their transcriptome.The results showed that the grafted seedlings with‘ZY-1'as rootstock had the lowest H_(2)O_(2) production,the highest activities of protective enzymes CAT,POD and SOD under high salt stress,and the net photosynthetic rate of the grafted seedlings decreased the least.So,compared with other materials,‘ZY-1'rootstocks have stronger salt resistance.At the same time,we sequenced the transcriptome of the grafted seedlings with‘ZY-1'as the rootstock under high salt stress,and obtained 51.52 Gb clean reads.The Q20 value of each sequenced sample was more than 97.66%,and the GC content was 41.39%.The sequencing results were feasible.Through database comparison,a total of 38861 clean data were annotated,including 763 and 2065 differentially expressed genes at 24 and 48 h of salt stress.After analyzing the biological functions of the differentially expressed genes,it is suggested that the genes involved in hormone signal transduction,starch and sucrose metabolism,ion transport and transcription factors may play an important role in the process of salt resistance in cherry.