期刊文献+

Aurora B kinase activation requires survivin priming phosphorylation by PLK1 被引量:2

原文传递
导出
摘要 During cell division,chromosome segregation is orchestrated by the interaction of spindle microtubules with the centromere.Accurate attachment of spindle microtubules to kinetochore requires the chromosomal passenger of Aurora B kinase complex with borealin,INCENP and survivin(SUR).The current working model argues that SUR is responsible for docking Aurora B to the centromere whereas its precise role in Aurora B activation has been unclear.Here,we show that Aurora B kinase activation requires SUR priming phosphorylation at Ser20 which is catalyzed by polo-like kinase 1(PLK1).Inhibition of PLK1 kinase activity or expression of non-phosphorylatable SUR mutant prevents Aurora B activation and correct spindle microtubule attachment.The PLK1-mediated regulation of Aurora B kinase activity was examined in real-time mitosis using fluorescence resonance energy transfer-based reporter and quantitative analysis of native Aurora B substrate phosphorylation.We reason that the PLK1-mediated priming phosphorylation is critical for orchestrating Aurora B activity in centromere which is essential for accurate chromosome segregation and faithful completion of cytokinesis.
出处 《Journal of Molecular Cell Biology》 SCIE CAS CSCD 北大核心 2011年第4期260-267,共8页 分子细胞生物学报(英文版)
基金 supported,in whole or in part,by National Institutes of Health(NIH)Grants DK-56292 and CA132389 and NIH,National Center for Research Resources(NCRR),Grant UL1 RR025008 from the Clinical and Translational Science Award Program supported by Chinese Natural Science Foundation Grants 30500183 and 30870990(to X.D.),and 90508002 and 90913016(to X.Y.) Chinese Academy of Science Grants KSCX1-YW-R-65,KSCX2-YW-H-10,KSCX2-YW-R-195 the Major State Basic Research Development Program of China(973 Program)2007CB914503 and 2010CB912103 International Collaboration Grant 2009DFA31010(to X.D.)and Technology Grant 2006BAI08B01-07(to X.D.) a Georgia Cancer Coalition Breast Cancer Research Grant Atlanta Clinical and Translational Science Award Chemical Biology Grant P20RR011104 and Anhui Province Key Project Grant,08040102005 supported in part by NCRR,NIH,Grant G12RR03034.
  • 相关文献

同被引文献6

引证文献2

二级引证文献8

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部