基于脂质组学探究代谢综合征小鼠的饮食干预

Lipidomics analysis of colon contents in metabolic syndrome mice with phospholipids of silver carp intervention

代谢综合征(MetS)的发病率正在增加,鲶鱼磷脂对降低MetS风险具有显著效果。采用拟靶向脂质组学分析了MetS小鼠结肠内容物中的脂质。通过建立代谢综合征模型将小鼠分为高脂和磷脂组进行不同的饮食干预、生化测量和脂质组学分析。在代谢方面发现6种差异代谢物,包括2种游离脂肪酸(FAs)、1种异丙醇脂类(PRs)、1种磷脂酰胆碱(GPs)和2种三酰甘油(TGs),可能是关键差异代谢物,2条代谢途径明显受到影响,分别是甘油脂代谢和甘油磷脂代谢;在机制方面,发现鲶鱼磷脂干预可以增加磷脂酰胆碱(PC)(34∶4)、亚油酸(LOA)(18∶2)和二十碳五烯酸(EPA)(20∶5),同时减少花生四烯酸的产生(5-iso PGF2VI),导致小鼠血脂、血糖、炎症因子和肾区脂肪的显著降低,有效缓解代谢综合征。

The incidence of metabolic syndrome(MetS)is increasing,and catfish phospholipids have a significant effect on reducing the risk of MetS.In this study,colonic contents lipids of MetS mice were analyzed using pseudo-targeted lipidomics.By establishing a metabolic syndrome model,mice were divided into high-fat and phospholipid groups with different diets,and biochemical measurements and lipidomic analysis were performed after the experiment.In terms of metabolism,6 differential metabolites,including 2 free fatty acids(FAs),1 isopropanolipid(PRs),1 phosphatidylcholine(GPs)and 2 triglycerides(TGs),may be the key differential metabolites and 2 metabolic pathways were significantly affected,namely glycerolipid metabolism and glycerophospholipid metabolism,and in terms of mechanism,catfish phospholipid intervention was found to increase phosphatidylcholine(PC)(34∶4),linoleic acid(LOA)(18∶2)and eicosapentaenoic acid(EPA)(20∶5),while decreasing the production of arachidonic acid(5-iso PGF2VI),resulting in significant reductions in blood lipids,blood glucose,inflammatory factors and perirenal lipids in mice,effectively alleviating the metabolic syndrome.