植物组培抗菌剂PPM对萝卜种子消毒及无菌苗生长的影响

Effects of plant preservative mixture(PPM)on seed disinfection and aseptic seedling growth of radish

为了建立萝卜组培过程中种子消毒及培养的简单有效方法,以萝卜种子为试材,采用L1(643)正交设计,开展了植物组培抗菌剂PPM浸泡浓度(1.0%、1.5%、2.0%和3.0%)、浸泡时间(0.0、0.5、2.0和4.0 h)及培养基中PPM浓度(0.0、0.1%、0.3%和0.6%)对萝卜种子消毒效果及无菌苗生长的研究。结果表明,PPM浸泡浓度对污染率有显著影响,对发芽率、根长、株高和下胚轴长的影响不显著;PPM浸泡时间对发芽率、污染率、根长、株高和下胚轴长均有显著影响;培养基中PPM浓度对污染率、根长、株高和下胚轴长具有显著影响,对发芽率无显著影响。主次效应分析表明,培养基中PPM浓度是影响污染率、根长、株高和下胚轴长的最主要因素,PPM浸泡时间影响次之,PPM浸泡浓度影响最小,最优组合A2B3C2可使发芽率达到96.67%,污染率为2.22%,根长为7.00 cm,株高为8.60 cm,下胚轴长为0.73 cm。探索开发了一套操作简便、无需冲洗的萝卜种子消毒方法以及高发芽率、低污染率的无菌苗培养方法。

In order to establish a simple and efficient method for seed disinfection and aseptic seedling culture in radish tissue culture,L16(43)orthogonal design,which including soaking concentration(1.0%、1.5%、2.0%and 3.0%),soaking time(0.0、0.5、2.0 and 4.0 h)and PPM(plant preservative mixture)concentration(0.0、0.1%、0.3%and 0.6%)in the culture medium,was used to study the disinfection effect of PPM on radish seeds and the growth of steriled seedlings.The results showed that the soaking concentration of PPM exhibited a significant effect on the contamination rate,but was not significant on the germination rate,root length,plant height and hypocotyl length;the soaking time of PPM had significant effect on germination rate,contamination rate,root length,plant height and hypocotyl length;the concentration of PPM added in the medium had a significant effect on the contamination rate,root length,plant height and hypocotyl length,but was not significant on the germination rate.The analysis of primary and secondary effects showed that the PPM concentration in culture medium was the most important factor affecting the contamination rate,root length,plant height and hypocotyl length,followed by the soaking time of PPM,and the soaking concentration of PPM was the smallest.The optimal combination A2B3C2 achieved a germination rate of 96.67%,a contamination rate of only 2.22%,a root length of 7.00 cm,a plant height of 8.60 cm,and a hypocotyl lenth of 0.73 cm.In summary,a set of simple and efficent methods with high germination rate and low contamination rate for radish seed disinfection and aseptic seedling culture are explored and developed.