死亡受体6在食管癌中表达及其与CCN1的关系研究

Expression of death receptor 6 in esophageal carcinoma and its relationship with CCN1

目的:研究死亡受体6(death receptor 6,DR6)在食管癌中的表达及细胞基质分子(cellular communication network factor 1,CCN1)对DR6表达的影响,寻找新的分子靶点,为食管癌的治疗提供帮助。方法:通过Real-Time PCR、免疫组织化学及Western Blot方法,分析食管肿瘤中DR6、细胞基质蛋白CCN1在食管癌中的表达,并通过细胞转染实验分析CCN1对DR6表达的影响。结果:DR6 mRNA在食管肿瘤细胞系中较食管正常上皮细胞表达升高(P<0.05);Western Blot及免疫组织化学分析显示DR6、CCN1在食管癌中均有表达,且在食管鳞状细胞癌中表达升高(P<0.05);CCN1上调了食管鳞癌、腺癌细胞株DR6的蛋白表达(P<0.05)。结论:在食管鳞状细胞癌中DR6、CCN1表达高于正常上皮细胞,可能参与食管鳞状细胞癌发生、发展。CCN1可以影响DR6的表达,进而可能影响DR6的生物学功能。

Objective:To study the expression of death receptor 6(DR6)in esophageal cancer and the effect of cellular communication network factor 1(CCN1)on the expression of DR6,and to find new molecular targets for the treatment of esophageal cancer.Methods:The expression of DR6 and CCN1 in esophageal cancer was analyzed by Real-Time PCR,immunohistochemistry and Western Blot.The effect of CCN1 on the expression of DR6 was analyzed by cell transfection experiment.Results:The expression of DR6 mRNA in esophageal cancer cell lines was higher than that in normal esophageal epithelial cells(P<0.05).Western Blot and immunohistochemical analysis showed that DR6 and CCN1 were both expressed in esophageal carcinoma and increased in esophageal squamous cell carcinoma(P<0.05).CCN1 up-regulated the protein expression of DR6 in esophageal squamous cell carcinoma and adenocarcinoma cell lines(P<0.05).Conclusion:The expression of DR6 and CCN1 in esophageal squamous cell carcinoma is higher than that in normal epithelial cells,which may be involved in the occurrence and development of esophageal squamous cell carcinoma.CCN1 can affect the expression of DR6,which may affect the biological function of DR6.