丹参不同生长时期叶片内抗氧化酶活性的比较研究

A Comparative Study on Antioxidant Enzyme Activities in the Leaves of Salvia Miltiorrhiza at Different Growth Stages

丹参是鼠尾草属植物,其根茎对于人类心脑血管疾病具有良好的治疗效果。本研究以紫花丹参为实验材料,对紫花丹参不同时期(营养生长期、花蕾期、开花期)叶片中的抗氧化酶(SOD、CAT、POD)活性进行比较。结果表明:丹参叶片在开花的过程中丙二醛(MDA)的含量在不断增大,由13.82 nmol/g增加到开花初期的23.39 nmol/g;同时叶片内的SOD活性也由3.09 U/g增加到36.56 U/g、CAT由1.08 U/(g·min^(-1))增加到7.58 U/(g·min^(-1)),POD由49.11 U/(g·min^(-1))降低到2 U/(g·min^(-1));可溶性蛋白含量由0.6 mg/g增加到2.01 mg/g。由此可以推断,丹参开花期的抗氧化酶活性高于营养生长期,且呈现增加趋势。可以对开花期进行适当的处理作为提升丹参产量的有效途径之一。

Salvia miltiorrhiza is a plant of the genus Salvia,and its rhizomes have good therapeutic effects on human cardiovascular and cerebrovascular diseases.In this study,Salvia miltiorrhiza is used as the experimental material to compare the activities of antioxidant enzymes(SOD,CAT,POD)in the leaves of salvia miltiorrhiza at different stages(nutritional growth period,bud stage,and flowering stage).The results illustrate that the content of malondialdehyde(MDA)increases continuously in salvia miltiorrhiza leaves during flowering,from 13.82 nmol/g to 23.39 nmol/g at the beginning of flowering.Meanwhile,the SOD activity in the leaves increases from 3.09 U/g to 36.56 U/g,CAT increased from 1.08 U/(g·min^(-1))to 7.58 U/(g·min^(-1)),POD declined from 49.11 U/(g·min^(-1))to 2 U/(g·min^(-1)).The soluble protein content increased from 0.6 mg/g to 2.01 mg/g.It can be concluded that the antioxidant enzyme activity of salvia miltiorrhiza at the anthesis stage is higher than that at the vegetative growth stage and presents a rising trend.The proper treatment at the flowering stage can be used as one of the effective ways to enhance the yield of salvia miltiorrhiza.