具有柔性和压电性的超薄Janus纳米纤维敷料用于伤口愈合

Ultrathin,flexible,and piezoelectric Janus nanofibrous dressing for wound healing

感染性皮肤伤口的愈合常常受到许多因素的影响,包括细菌感染、过量的伤口渗出物、局部灌注不良和细胞招募不足.开发有效管理伤口和促进伤口愈合的敷料是一个巨大的挑战.因此,我们通过逐层静电纺丝技术构建了一种具有抗菌性、柔性和压电特性的超薄Janus纳米纤维敷料.敷料的亲水层由随机排列的聚己内酯/明胶纳米纤维组成,而疏水层则由Ag纳米颗粒掺杂的聚偏氟乙烯有序纤维组成.体外和体内实验研究结果表明,Janus敷料不仅可以将过量的伤口渗出物单向排出,杀死局部细菌,还可以在正常身体运动下产生动态压电信号,促进成纤维细胞的增殖和迁移、胶原蛋白的沉积、血管生成和再上皮化,从而加速小鼠伤口的快速愈合.这种智能Janus敷料将为加速伤口愈合和伤口管理提供一种新方法.

The healing process of infected skin lesions is often delayed by many factors including bacterial infection,excessive accumulation of wound exudate,poor local perfusion,and low cell recruitment.Development of wound dressing for efficient wound management and promotion of wound healing is a great challenge.Herein,we constructed a Janus nanofiber dressing with the comprehensive capacities of bacterial killing,piezoelectrical stimulation for promoting fibroblast migration,and wound exudate removal via unidirectional liquid delivery.The rationally designed Janus nanofibrous dressing with ultrathin,flexible,breathable,and piezoelectric characteristics is fabricated via the facile layerby-layer electrostatic spinning technology.The hydrophilic layer of the dressing is composed of randomly arranged polycaprolactone/gelatin(PCL/Gel)nanofibers,while the hydrophobic layer consists of well-aligned Ag nanoparticles(Ag NPs)-doped piezoelectric polyvinylidene fluoride(PVDF/Ag)nanofibers.The in vitro and in vivo experimental results demonstrate that the Janus dressing can not only drain excess wound exudate out of the wound unidirectionally and kill local bacteria,but also generate dynamic piezopotential under normal body motions to promote fibroblast proliferation and migration,collagen deposition,angiogenesis,and re-epithelialization,which accelerates rapid wound healing on mice.The smart Janus dressing will provide a new approach for acceleration of wound healing and wound management.