体检人群幽门螺旋杆菌抗体定量检测及免疫分型的研究分析

Research and analysis of quantitative detection of Helicobacter pylori antibody and immunophenotyping in physical examination population

目的研究体检人群幽门螺旋杆菌(Helicobacter pylori,Hp)的感染及免疫分型情况。方法采用免疫比浊法对Hp抗体进行定量检测和免疫印迹方法对Hp抗体进行分型,分析不同性别、年龄的感染与免疫分型结果,以及感染抗体数与抗体水平的相关性。结果体检人群Hp总阳性率为41.37%(235/568),其中HpⅠ型阳性率为28.70%(163/568),HpⅡ型阳性率为12.68%(72/568)。不同性别的Hp感染率差异无统计学意义(χ^(2)=0.975,P=0.323),其中不同性别的HpⅠ型、HpⅡ型感染率差异均无统计学意义(P>0.05),不同性别的CagA、VacA、UreA、UreB抗体感染率差异均无统计学意义(P>0.05)。随着年龄的增加,Hp感染率、HpⅠ型感染率、CagA抗体感染率均有升高趋势(P<0.05);其中Hp感染率、HpⅠ型感染率、CagA抗体感染率的41岁~50岁和51岁~71岁感染率均高于18岁~30岁的感染率(P<0.05)。结论性别对Hp感染率无影响;高年龄段41岁~50岁和51岁~71岁的Hp感染率、HpⅠ型感染率、CagA抗体感染率较高。感染抗体数量对Hp抗体水平有影响。

Objective This paper aims to study the infection and immune typing of Helicobacter pylori(Hp)in physical examination population.Methods Quantitative detection of Hp antibody by immunoturbidimetry and typing of Hp antibody by immunoblotting were used to analyze the results of infection and immunotyping in different sex and age,as well as the correlation between the number of infected antibody and the level of antibody.Results The total positive rate of Hp was 41.37%(235/568),of which the positive rate of Hp type Ⅰ was 28.70%(163/568),and the positive rate of Hp type Ⅱ was 12.68%(72/568).There was no statistical significance on the difference in Hp infection rate between different sexes(χ^(2)=0.975,P=0.323),among which there was no statistical significance on the difference in the infection rates of HpⅠ and HpⅡ in different sexes(P>0.05),and there was no statistical significance on the difference in the infection rates of CagA,VacA,UreA and UreB antibodies in different sexes(P>0.05).Hp infection rate,HpⅠinfection rate and CagA antibody infection rate all increased with age(P<0.05).The infection rates of Hp,HpⅠand CagA antibody in 41-50 years old and 51-71 years old were significantly higher than those in 18-30 years old(P<0.05).Conclusion Sex has no effect on the HpⅠ infection rate;HpⅠinfection rate,HpⅠinfection rate and CagA antibody infection rate in the high age group of 41-50 and 51-71 years old were high.The number of infected antibodies has an impact on the level of Hp antibody.