超声波辅助提取美人蕉叶多酚工艺及其抗氧化活性研究

Ultrasonic⁃Assisted Extraction of Polyphenols From Canna indica Leaves and Its Antioxidant Activity

以美人蕉叶为原料,通过单因素试验并结合响应面法,对超声波辅助乙醇溶液提取美人蕉叶多酚的工艺条件进行研究.选择乙醇浓度、液料比、超声温度和超声时间作为响应面因素,多酚提取率为响应值,确定了能够用于美人蕉叶多酚提取率进行分析和预测的二次多项式回归模型方程.结果表明,在乙醇浓度为57%、液料比为43 mL/g、超声温度为72℃、超声时间为50 min时,测得的美人蕉叶多酚提取率最高,达到18.89 mg/g,与理论上的美人蕉叶多酚提取率(19.15 mg/g)相对误差仅为1.36%,证实了该模型合理,预测可靠.所提取的美人蕉叶多酚对DPPH自由基和OH自由基具有一定的清除能力并呈现正相关关系,其IC50分别为49.99 mg/L和315.55 mg/L,证明了美人蕉叶多酚的抗氧化能力.

Taking Canna indica leaves as raw materials,the process conditions of ultrasonic⁃assisted ethanol solution extraction of polyphenols from Canna indica leaves are studied by single factor experiment combined with response surface methodology.The ethanol concentration,liquid⁃to⁃material ratio,ultrasonic temperature and ultrasonic time are selected as the response surface factors,and the polyphenol extraction yield is the response value,and the quadratic polynomial regression model equation that can be used to analyze and predict the polyphenol extraction yield of Canna indica leaves is determined.The results show that when the concentration of ethanol is 57%,the ratio of liquid to material is 43 mL/g,the ultrasonic temperature is 72℃and the ultrasonic time is 50 minutes,the extraction yield of polyphenols from Canna indica leaves is the highest,reaching 18.89 mg/g.The theoretical relative error of Canna indica leaves polyphenol extraction yield(19.15 mg/g)is only 1.36%,which also confirms that the model is reasonable and the prediction is reliable.The extracted polyphenol from Canna indica leaves has a certain scavenging ability on DPPH free radical and OH free radical,and there is a positive correlation.Its IC50 is 49.99 mg/L and 315.55mg/L,respectively,which proves the antioxidant capacity of Canna indica leaves polyphenols.