基于脂质组学的敏感性皮肤表面脂质差异分析

Differential analysis of sensitive skin surface lipids based on lipidomics

建立了基于脂质组学技术的人面部皮肤表面脂质采集、检测及分析方法,用于敏感性皮肤脂质代谢紊乱的研究。首先通过乳酸刺痛实验将青年女性志愿者分为敏感性皮肤组和非敏感性皮肤组,检测其生理指标并采集面部皮肤表面脂质进行超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF-MS)分析。随后,运用正交偏最小二乘-判别分析(OPLS-DA)结合t检验筛选出差异性成分并进行精准二级质谱鉴定,共指认出29种甘油三酯类(TG)差异性脂质。与非敏感性皮肤组相比,敏感性皮肤组中这些差异性TG的水平显著降低,并对相关生物学意义进行了阐述。本研究为敏感性皮肤发生机制的探索及相关护肤品的研发提供了理论指导和技术支撑。

This paper established a method for collecting,detecting,and analyzing the human facial skin surface lipids based on lipidomics technology for the study of lipid metabolic disorders in sensitive skin.Firstly,the young female volunteers were divided into the sensitive skin group and non-sensitive skin group by lactic acid stinging test,then the physiological indices were detected and the facial skin surface lipids were analyzed by the ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry(UPLC-Q-TOF-MS).Subsequently,the differential components were screened by orthogonal partial least-squares discrimination analysis(OPLS-DA)combined with t test and identified via high-resolution MS/MS,and totally 29 differential lipids of triglyceride(TG)were assigned.Compared with the non-sensitive skin group,the levels of these differential TGs are significantly reduced in the sensitive skin group,and the relevant biological significance is explained.This study provides theoretical guidance and technical support for the exploration of the pathogenesis of sensitive skin as well as the research and development of related skin care products.