蚕丝丝肽嗜热栖热菌发酵液对皮肤抗炎抗衰功效的增效作用

Synergistic effect of silk peptides thermophilus fermentation on the skin anti-inflammatory and anti-aging activities

为研究嗜热栖热菌发酵的蚕丝丝肽在皮肤抗炎抗衰功效上的增效作用,以脂多糖(LPS)诱导的角质形成细胞(HaCaT细胞)和双氧水(H2O2)诱导的成纤维细胞(HDF细胞)为研究模型,通过比色法、酶联免疫吸附(ELISA)等方法检测蚕丝丝肽、嗜热栖热菌发酵液和蚕丝丝肽发酵液对细胞活力、炎症因子白介素1β(IL-1β)和白介素6(IL-6)的含量、屏障保湿相关蛋白水通道蛋白3(AQP3)和丝聚蛋白(FLG)的含量、衰老相关标志物超氧化物歧化酶(SOD)、晚期糖基化终产物(AGEs)和人1型胶原蛋白(Col-1)的含量变化的影响。结果表明,蚕丝丝肽发酵液能够显著降低IL-1β,IL-6,AGEs含量,显著促进FLG,AQP3,SOD和Col-1表达,且相较于蚕丝丝肽能更显著抑制IL-6和AGEs表达,更显著促进HDF细胞迁移和SOD合成,而相较于嗜热栖热菌发酵液能更显著降低IL-1β,IL-6和AGEs表达,更显著促进AQP3表达、HDF细胞迁移和SOD的合成,即认为蚕丝丝肽发酵液相较于单一的蚕丝丝肽和嗜热栖热菌发酵液具备抗炎、屏障保湿及抗衰功效的增效作用。

In order to study the synergistic effects of silk peptide fermented by thermophilus on skin anti-inflammatory,barrier moisturizing and anti-aging activities,silk peptides thermophilus fermentation was prepared referring to the method of microbial fermentation,and lipopolysaccharide(LPS)-induced keratinocytes(HaCaT cells)and hydrogen peroxide(H2O2)-induced fibroblasts(HDF cells)were used as research models.The inhibition rates of cell proliferation,the contents of interleukin 1β(IL-1β),interleukin 6(IL-6),aquaporin 3(AQP3),filaggrin(FLG),superoxide dismutase(SOD),advanced glycosylation end products(AGEs)and human collagen I alpha 1(COL-1)were detected by colorimetric assay,enzyme-linked immunosorbent assay(ELISA),et al.The results show that silk peptide fermentation can significantly reduce the expression of IL-1β,IL-6 and AGEs,and can significantly promote the expression of FLG,AQP3,SOD and COL-1.Especially the silk peptide fermentation can more significantly inhibit the expression of IL-6 and AGEs,and can more significantly promote the migration of HDF cells and SOD synthesis than the single silk peptide.And also the silk peptide fermentation can more significantly reduce the expression of IL-1β,IL-6 and AGEs,and can more significantly promote the expression of AQP3,HDF cell migration and SOD synthesis than the single fermentation of thermophilic bacteria.Compared with the single silk peptide and the fermentation of thermophilus,the silk peptide fermentation has obvious synergistic effects of antiinflammatory,barrier moisturizing and anti-aging activities.