千里光化学成分及抗烟草花叶病毒活性研究

Chemical constituents of Senecio scandens and their anti-tobacco mosaic virus activity

对千里光(Senecio scandens)化学成分及其抗烟草花叶病毒(TMV)活性进行研究,为开发新型植物病毒抑制剂提供理论依据。综合运用硅胶、凝胶、MCI等多种柱层析方法对千里光地上部分乙酸乙酯萃取物化学成分进行分离,利用NMR、MS鉴定其结构;采用活体半叶枯斑法测定化合物对TMV的抑制活性。从千里光乙酸乙酯萃取物中共分离得到13个化合物,根据其理化性质以及波谱数据分别鉴定为24-烯-环阿尔廷酮(1)、3β-羟基-7β-甲氧基-5-豆甾烯(2)、正三十二烷(3)、乌索酸(4)、豆甾醇(5)、(22 E)-ergosta-6,22-diene-3β,5β,8α-triol(6)、山柰酚(7)、3-吲哚甲醛(8)、泽兰黄酮(9)、槲皮素(10)、紫杉叶素(11)、jacaranone(12)、叶绿醇(13)。除化合物7和化合物10外,其余化合物均为首次从该植物中分离得到。活性测定结果表明,化合物8和化合物13具有较强的抗TMV活性。

In order to provide a theoretical basis for the development of new plant virus inhibitors,the chemical components of Senecio scandens and its activity against tobacco mosaic virus(TMV)were determined.The chemical constituents of the ethyl acetate extract from the aboveground part of S.scandens were separated by silica gel,Sephadex LH-20,MCI and other column chromatography methods,and its structures were identified by NMR and MS data.The inhibitory activity of the compound against TMV was screened by the half-leaf spot method in vivo.Thirteen compounds were isolated from the ethyl acetate extract of S.scandens.According to their physicochemical properties and spectral data,they were identified as 9,19-cyclolanost-24-en-3-one(1),7β-methoxystigmast-5-en-3β-ol(2),dotriacontane(3),ursolic acid(4),stigmasterol(5),(22 E)-ergosta-6,22-diene-3β,5β,8α-triol(6),kaempferol(7),indole-3-carboxaldehyde(8),nepetin(9),quetrcetin(10),taxifolin(11),jacaranone(12),phytol(13).Except compound 7 and compound 10,the other compounds were isolated from this plant for the first time.The results showed that compound 8 and compound 13 had strong anti-TMV activity.