行波离子迁移谱技术及应用研究进展

Advancement of Traveling Wave Ion Mobility Spectrometry and Its Application

离子迁移谱(ion mobility spectrometry,IMS)是利用离子迁移率K(离子碰撞截面)差异来实现不同离子的分离与测定,具有分析速度快、检测灵敏度高的优点,其与质谱联用在蛋白质组学、代谢组学、医药等领域已获得了广泛的应用.随着分析对象复杂性的增加,对IMS的分辨能力也提出了更高要求.行波离子迁移谱(travelling wave ion mobility spectrometry,TWIMS)采用时域连续的行波电场实现离子传输与分离,其分析通道的长度不受行波电压幅值的限制,理论上可以无限延长离子分析通道来提高分辨能力.目前,TWIMS的分辨率最高可达1860,对于分析存在多种同分异构体的复杂样品别具优势.对TWIMS的原理及分辨能力的影响因素进行了介绍,进一步探讨了不同结构TWIMS仪器的特点、性能和应用,对TWIMS未来发展方向进行了展望.

Ion mobility spectrometry(IMS)utilizes the difference in ion mobility K(collision cross section)to realize the separation and determination of different ions,which has the advantages of fast analysis speed and high sensitivity.And it coupling with mass spectrometry(IM-MS)was widely used in the fields of proteomics,metabolomics,medicine,etc.With the increasing complexity of the analyzed objects,higher demands are put on the resolution of the IMS.Traveling wave ion mobility spectrometry(TWIMS)uses a time-domain continuous traveling wave electric field to realize ion transport and separation.The analytical path length of the TWIMS is not limited by the amplitude of the travelling wave voltage,theoretically the path can be extended indefinitely to improve the resolution.Currently,the resolution of TWIMS can reach up to 1860,which is advantageous for the analysis of complex samples with the multiple isomers.The principle of TWIMS and the influencing factors of resolution were introduced,the characteristics,performance and applications of TWIMS instruments with different structures were further discussed,and finally the future development directions of TWIMS were prospected.