基于镍钼硫化物纳米花修饰碳纳米管的脑利钠肽电化学免疫传感器的构建

Construction of Electrochemical Immunosensor for Ultrasensitive Determination of Brain Natriuretic Peptide Based on Nanocomposite of Nickel Molybdenum Sulfide Supported on Carbon Nanotubes

制备了一种三维多孔镍钼硫化物纳米花修饰碳纳米管(CNT)的复合材料(NiMoS@CNT).通过扫描电子显微镜和X射线衍射表征所合成材料的形貌和结构.利用循环伏安法和计时电流法对所制备材料的电化学催化性能进行研究.基于NiMoS@CNT对过氧化氢(H2O2)优异的电催化性能,构建了一种检测脑利钠肽的夹心型电化学传感器.在最优条件下,电流响应强度和脑利钠肽质量浓度的对数在0.20~20ng/mL范围内呈线性关系.结果表明免疫传感器具有高的灵敏度、选择性和稳定性,可用于实际样品的检测.

A three-dimensional porous nickel molybdenum sulfide nanoflowers supported on carbon nanotubes(CNT)composite materials(NiMoS@CNT)was prepared.The morphology and structure of the synthesized materials were characterized by scanning electron microscopy and X-ray diffraction.The cyclic voltammetry and chronoamperometry were used to study the electrochemical catalytic performance of the prepared materials.Based on the excellent electrocatalytic performance of NiMoS@CNT for hydrogen peroxide,a sandwich electrochemical sensor for detecting brain natriuretic peptide(BNP)was constructed.Under the optimal conditions,the current response intensity and the logarithm of mass concentration of brain natriuretic peptide was linear in the range of 0.20~20 ng/mL.The immunosensor has a high sensitivity,selectivity and stability,and can be used for the detection of BNP in actual samples.