顶空气相色谱-电子捕获检测器法测定明胶空心胶囊中2-氯乙醇残留量

Determination of 2-Chloroethanol Residues in Gelatin Hollow Capsules by Headspace Gas Chromatography-Electron Capture Detector Method

建立明胶空心胶囊中2-氯乙醇残留量的顶空气相色谱-电子捕获检测器分析测定方法.明胶空心胶囊溶液经NaCl溶液盐析后失去明胶的物理性质,采用顶空气相色谱-电子捕获检测器对明胶空心胶囊溶液中2-氯乙醇含量进行分析.试验结果表明,2-氯乙醇在10~35µg/g范围内呈良好线性关系,r为0.9994,定量限和检出限分别为10和5µg/g,回收率为92.1%~102.2%,相对标准偏差(RSD)均小于10%,使用所建立的方法对样品进行了检验.方法简单准确,灵敏度高,有效解决了明胶空心胶囊基质效应,可用于市售明胶空心胶囊中2-氯乙醇的定量分析.

A method was developed to determine the residues of 2-chloroethanol in gelatin hollow capsules using headspace gas chromatography-electron capture detector method.The physical properties of gelatin disappeared when the gelatin hollow capsule solution was salting out with NaCl solution.The content of 2-chloroethanol in gelatin hollow capsule solution was analyzed by headspace gas chromatography-electron capture detector method.The results showed that 2-chloroethanol had a good linear relationship in the range of 10~35µg/g,with an r value of 0.9994.The limits of quantitation and limits of detection were 10 and 5µg/g,respectively.The recoveries ranged from 92.1%~102.2%,the relative standard deviation(RSD)were all less than 10%.The samples were tested by the method.The method is simple,accurate and sensitive and effective in solving the matrix effect of gelatin hollow capsules,and can be used for the quantitative analysis of 2-chloroethanol in commercially available gelatin hollow capsules.