肠道主要产丁酸细菌与内脏脂肪和胰岛素抵抗的相关性

Association of predominant butyrate-producing bacteria with visceral adipose tissue and insulin resistance

目的 探讨4种肠道主要产丁酸细菌与内脏脂肪和和胰岛素抵抗的关系,为后续研究提供参考。方法 应用实时荧光定量PCR检测胰岛素抵抗组(76例,HOMA-IR≥2.68)和健康对照组(273例,HOMAIR<2.68)对象4种肠道主要产丁酸细菌的丰度变化。采用生物电阻抗法测量所有研究对象的内脏脂肪面积(visceral fat area,VFA)和体脂肪百分比(body fat percentage,BFP)。运用ROC曲线比较VFA和BFP对胰岛素抵抗的诊断价值。结果 VFA的ROC曲线下面积(0.751)显著高于BFP(0.647)。胰岛素抵抗组和健康对照组对象肠道4种产丁酸细菌的丰度差异均无统计学意义(均P>0.05)。Spearman相关分析显示普拉梭菌(R=-0.106,P<0.05)和柔嫩梭菌(R=-0.115,P<0.05)与HOMA-IR呈负相关。VFA与普拉梭菌呈负相关(R=-0.105,P<0.05),BFP与普拉梭菌和柔嫩梭菌均无显著相关性。调整BMI和年龄因素后,回归分析显示普拉梭菌与甘油三酯和空腹血糖独立相关,柔嫩梭菌与尿酸独立相关。结论 VFA对胰岛素抵抗的诊断价值比BFP高。普拉梭菌和柔嫩梭菌与胰岛素抵抗存在相关性,且普拉梭菌与内脏脂肪相关。

Objective To explore the association of four predominant butyrate-producing bacteria with visceral adipose tissue and insulin resistance,providing a reference for following research.Methods This cross-sectional study included 349 individuals,who were divided into insulin resistance group(76 cases,HOMA-IR≥2.68)and healthy control group(273 cases,HOMA-IR<2.68).The q-PCR was used to detect counts of the four predominant butyrate-producing bacteria.The bioimpedance analyzer was used to measure visceral fat area(VFA)and body fat percentage(BFP).The receiver operating characteristic curves(ROC curves)was used to estimate the diagnostic accuracy of VFA,BFP,BMI for the HOMAIR index.Results The area under the curve(AUC)for VFA(0.751)was higher than BFP(0.647).There was no significant difference in the counts of the four bacteria between two groups(all P>0.05).F.prausnitzi(R=-0.106,P<0.05)and C.leptum(R=-0.115,P<0.05)were negatively correlated with HOMA-IR.F.prausnitzi was negatively correlated with VFA(R=-0.105,P<0.05).BFP was not related to either of these two meaningful bacteria.After adjustment for BMI and age,F.prausnitzii was independently associated with TG and FBG,and C.leptum was independently associated with uric acid.Conclusion The diagnostic accuracy of VFA for the HOMA-IR was higher than BFP.There is an association between F.prausnitzi and C.leptum and insulin resistance.Moreover,F.prausnitzi was correlated with visceral adipose tissue.