跌打万应膏治疗踝关节软组织损伤的实验研究

Experimental Study of Diedawanying Ointment on the Treatment of Ankle Joint Soft Tissue Injury

目的:探讨跌打万应膏治疗踝关节软组织损伤的作用机制。方法:选取雄性大鼠72只,称重,随机分为6组,每组12只,分别为空白组、模型对照组、跌打万应膏高、中、低剂量组、对照组(冰敷),空白组不用药,其他组按体重各自给药。采用经典的重锤坠落法造模建立大鼠踝关节急性软组织损伤模型,观察各组大鼠第3天和第7天后血清炎性递质IL-1β、TNF-α的水平以及应用荧光聚合酶链式反应(PCR)及蛋白质免疫印迹技术(Western Blot)检测各组大鼠受损软组织TLRs/MyD88/NF-κB通路表达水平的变化。结果:跌打万应膏可降低外周血中的IL-1β、TNF-α炎症因子水平,并显著抑制TLRs/MyD88/NF-κB信号通路TLR2、TLR4、MyD88、NF-κB基因及蛋白的激活和表达。结论:跌打万应膏外敷以中剂量疗效为佳,TLRs/MyD88/NF-κB信号通路可能是跌打万应膏的作用途径之一。

Objective:To investigate the mechanism of diedawanying ointment on the treatment of ankle soft tissue injury.Methods:72 male rats were selected and weighed and randomly divided into 6 groups,with 12 rats in each group,which were blank group,model control group,diedawanying ointment high-dose,medium-dose and low-dose groups,and control group(ice compress).Blank group was not given medicine,and other groups were given medicine according to body weight.Acute soft tissue injury model of the ankle joint in rats was established by the classical hammer fall method.The levels of serum inflammatory transmitters IL-1βand TNF-αwere observed after 3 d and 7 d in each group,and the expression levels of TLRs/MyD88/NF-κB pathway in the damaged soft tissues of rats were detected by PCR and Western Blot.Results:Diedawanying ointment could decrease the levels of IL-1βand TNF-αinflammatory factors in peripheral blood,and significantly inhibit the activation and expression of TLR2,TLR4,MyD88 and NF-κB genes and proteins in TLRs/MyD88/NF-κB signaling pathway.Conclusion:Medium dose of diedawanying ointment has the best therapeutic effect.The action mechanism of diedawanying ointment is complex,and TLRs/MyD88/NF-κB signaling pathway is one of the action pathways of diedawanying ointment.