促进蛋白质结晶的方法研究进展

Technological Advances in Promoting Protein Crystallization

蛋白质结晶对蛋白质的提纯、保存以及结构解析等具有重要意义。由于蛋白质结晶易受到物理、化学和生物等多种因素的影响,导致蛋白质晶体形成过程不易调控,结晶效率随机并且结晶产率低,限制了蛋白质结晶的实际应用。因此,研究促进蛋白质结晶的方法不仅可以提高蛋白质结晶效率及结晶过程的可控性,还能够有效推动蛋白质结构解析及药物开发等领域的发展。本文总结了促进蛋白质结晶的代表性方法与技术,分类介绍了不同方法相关的机制,并对促进蛋白质结晶的研究方法进行了分析和展望。

Protein crystallization is important for the purification,preservation and structural study of protein.Since protein crystallization is susceptible to physical,chemical and biological factors,the process of protein crystal formation is difficult to regulate,the crystallization efficiency is random,and the crystallization yield is low,which limits the practical application of protein crystallization.Therefore,the study of method for promoting protein crystallization can not only improve the efficiency and controllability of protein crystallization process,but also effectively advance the fields of protein structure analysis and drug development.This paper summarizes the representative methods and techniques for promoting protein crystallization,classifies the mechanisms associated with various approaches,and analyzes and forecasts the research methods for promoting protein crystallization.