抑郁症大鼠模型构建与评价

Construction and Evaluation of Depression Rat Model

目的:通过慢性不可预知的温和应激法(CUMS)建立大鼠抑郁症模型。方法:将SD雄性大鼠12只随机分为正常对照组和模型(CUMS)组,每组6只。对CUMS组大鼠进行连续50天的慢性不可预知温和刺激,并最终通过糖水偏好实验和旷场实验的不动时间进行评价。结果:CUMS组大鼠的糖水偏好率(0.257833±0.052285)明显低于正常组的(0.732167±0.046210)(P<0.01);CUMS组大鼠的旷场实验不动时间(348.333333±8.165)明显长于正常组的(174.333333±6.121)(P<0.01)。CUMS组大鼠造模后的糖水偏好率(0.257833±0.052285)明显低于造模前(0.611667±0.062981)(P<0.05);CUMS组大鼠造模后的旷场实验不动时间(348.333333±8.165)明显高于造模前(218.166667±46.820580)(P<0.05)。结论:通过慢性不可预知的温和应激法(CUMS)成功建立抑郁症大鼠模型。

Objective:To establish depression rat model by chronic unpredictable mild stress(CUMS).Methods:12 male SD rats were randomly divided into normal control group and model group(CUMS),with 6 rats in each group.The CUMS group rats were subjected to chronic unpredictable mild stimulation for 50 consecutive days,and finally evaluated by the fixed time of sugar water preference test and open field test.Results:The sugar preference rate of rats in CUMS group(0.257833±0.052285)was significantly lower than that in normal group(0.732167±0.046210)(P<0.01);The immobility time of rats in the CUMS group(348.33333±8.165)was significantly longer than that in the normal group(174.333333±6.121)(P<0.01).The sugar-water preference rate of rats in CUMS group(0.257833±0.052285)after modeling was significantly lower than that before modeling(0.611667±0.062981)(P<0.05);The immobility time of the open field experiment in the CUMS group after modeling(348.33333±8.165)was significantly higher than that before modeling(218.166667±46.820580)(P<0.05).Conclusion:The depression rat model was successfully established by chronic unpredictable mild stress method(CUMS).