气相色谱法测定清肠栓中樟脑残留量和冰片含量

Determination of camphor residue and borneol content in Qingchang suppository by GC

目的建立清肠栓中樟脑残留物和冰片含量的气相测定方法。方法采用气相色谱法,色谱柱为Agilent毛细管柱(30 m×0.25 mm×0.25µm),柱温为140℃,进样口温度250℃,检测器(FID)温度为250℃。结果樟脑、龙脑和异龙脑在0.0299~1.497µg(r=1.000)、0.0205~1.025µg(r=1.000)、0.0097~0.4830µg(r=1.000)范围内呈现良好的线性关系。精密度、稳定性、重复性试验结果的RSD均小于2%,平均加样回收率分别为99.7%、101.0%、102.5%。结论该方法准确、可靠、简便快速,可用于清肠栓中龙脑含量的测定。

Objective To establish a gas chromatography for simultaneous determination of camphor residue and borneolum content in Qingchang Suppository.Methods Gas chromatograph method was used.The chromatographic column was Agilent capillary column(30 m×0.25 mm×0.25μm).The column temperature was 140℃.The sample injection temperature was 250℃.The FID detector temperature was 250℃.Results Camphor,borneol and isoborneol content showed good linear in the extent of 0.0299~1.497(r=1.000),0.0205~1.025(r=1.000),0.0097~0.4830μg(r=1.000).RSDs of precision,stability and repeatability test results were less than 2%.The recovery was 99.7%,101.0%,102.5%.Conclusion This method is simple and quick with accurate result,which could be used for the content determination of Borneol in Qingchang Suppository.