牛血清白蛋白@席夫碱双荧光化合物组合构建三输入-双输出分子逻辑电路

Desingning triple-input-double-output molecular logic circuits based on the dual fluorescent compound of BSA@Schiff base

基于牛血清白蛋白与邻苯二胺缩二5-氟水杨醛这两种荧光化合物组合的方式设计了一种荧光探针,并采用荧光光谱、同步荧光光谱、三维荧光光谱及分子对接等方法研究它们之间相互作用的机理。实验结果表明,邻苯二胺缩二5-氟水杨醛与牛血清白蛋白之间可以形成稳态复合物,在形成过程中氢键及范德华力起主导作用,且邻苯二胺缩二5-氟水杨醛对牛血清白蛋白的构象会产生影响。此外,在280nm激发下,邻苯二胺缩二5-氟水杨醛与牛血清白蛋白之间可以基于荧光共振能量转移而在350nm及460nm处产生两个荧光发射峰。基于该特征,文章以牛血清白蛋白@邻苯二胺缩二5-氟水杨醛作为分子基逻辑材料,通过不同的金属离子对两个荧光发射峰进行调节,从而成功地构建出三个不同的分子逻辑路。

A fluorescent probe based on the combination of BSA and o-phenylenediamine-5-fluorosalicylaldehyde was designed,and the mechanism of their interactions using fluorescence spectroscopy,simultaneous fluorescence spectroscopy,three-dimensional fluo-rescence spectroscopy and molecular docking was in vestigated.The experimental results show that a steady-state complex can be formed between o-phenylenediamine-5-fluorosalicylaldehyde and BSA,with hydrogen bonding and van der Waals forces playing a dominant role in the formation process,and that o-phenylenediamine-5-fluorosalicylaldehyde have an effect on the conformation of BSA.In addition,two fluorescence emission peaks at 350 nm and 460 nm can be generated between o-phenylenediamine-5-fluoro-salicylaldehyde and BSA based on fluorescence resonance energy transfer under 280 nm excitation.Based on this feature,three dif-ferent molecular logic pathways were successfully constructed by modulating the two fluorescence emission peaks with different metal ions using BSA@o-phthalimide-5-fluorosalicylaldehyde as the molecular-based logic material.