银杏叶提取物等5种药物抑制蛋白质体外糖基化作用的比较

Comprarison of inhibitory effects on nonenzymatic glycosylation of protein in vitro by Ginkgo biloba extract and other four drugs

目的 :研究银杏叶提取物、葛根素、氨基胍、二甲双胍和普罗布考对蛋白质体外非酶糖基化反应的影响。方法 :分别将上述 5种药物与葡萄糖和牛血清清蛋白孵育 ,并在 4 ,8,12wk时取出 ,用荧光法测定糖基化产物的自发荧光值。结果 :银杏叶提取物组、葛根素组、氨基胍组均能抑制蛋白质体外非酶糖基化在 5 0mmol·L- 1葡萄糖组分别为 (5 .6±s 0 .4 ) ,(8.3± 0 .7) ,(6 .0± 0 .6 )AFU ,与对照组(11.0± 1.0 )AFU比较 ,差异有非常显著意义 (P <0 .0 1) ,其中银杏叶提取物组的抑制作用比氨基胍组更明显。二甲双胍组与对照组相比 ,糖基化产物的荧光值有降低趋势 ,但差异未达到显著水平。普罗布考组对蛋白质糖基化无影响。结论 :银杏叶提取物和葛根素可明显抑制蛋白质体外糖基化反应 。

AIM: To compare inhibitory effect of Ginkgo biloba extract(GbE), puerarin, amino-guanidine, metformin and probucol on the nonenzymatic glycosylation of protein in vitro. METHODS: D-glucose and bovine serum albumin (BSA) were incubated with five drugs respectively for 4, 8, 12 wk. The fluorescence intensity of glycosylated BSA was detected by a spectrophotometer. RESULTS: The glycosylation of protein in vitro was inhibited in GbE, puerarin and aminoguanidine groups and those were (5.6±s 0.4),(8.3± 0.7), (6.0±0.6)AFU,respectively, compared with control group (11.0±1.0)AFU, (P < 0.01); moreover, the rate of inhibition in GbE group was higher than that of aminoguanidine group. Metformin group compared with control group the glycosylation of protein seemingly reduced, but difference was not significant. Probucol group had no effect on glycosylation in vitro. CONCLUSION: GbE and puerarin inhibit nonenzymatic glycosylation of protein in vitro, they might be useful in the treatment of diabetes and its vascular complications prospectively.