KIF20A表达在膀胱癌中的预后作用及与肿瘤免疫浸润的关系

Prognostic effect of KIF20A expression in bladder cancer and its relationship with tumor immune infiltration

目的 研究KIF20A在膀胱癌中的表达情况,进一步分析其对肿瘤预后和免疫微环境的影响。方法 本研究基于生物信息学方法和R语言统计软件。收取TCGA、GEO、ArrayExpress公共数据库2004年6月—2022年10月的1 145例膀胱癌及183例正常膀胱组织的测序样本,分析KIF20A的mRNA表达水平。结合TCGA膀胱癌队列的临床病理参数和预后随访数据,利用卡方分析和Kaplan-Meier生存分析探究KIF20A表达量与膀胱癌临床病理特征和总体预后的关系。随后通过TIMER算法评估肿瘤免疫细胞丰度,探究肿瘤KIF20A表达与免疫细胞浸润的关系。结果 综合全球1 145例膀胱癌及183例正常膀胱组织的RNA测序样本,KIF20A在肿瘤中显著高表达(P<0.05),标准平均差(SMD)为1.10。并且通过KIF20A的表达量可以区分肿瘤和正常组织。然而,生存分析显示KIF20A的表达量与膀胱癌患者预后无显著关联。在肿瘤免疫微环境探究中,发现树突状细胞、CD8+T细胞、中性粒细胞和巨噬细胞的浸润水平与KIF20A表达相关。结论 KIF20A在膀胱癌组织中过表达,这与肿瘤微环境中免疫细胞的浸润减少相关联。KIF20A是膀胱癌免疫治疗新的潜在靶标。

Objective To study the expression of KIF20A in bladder cancer,and further analyze the effect of KIF20A on tumor prognosis and immune microenvironment.Methods This study was based on bioinformatics method and R software.RNA sequencing samples of 1,145 cases of bladder cancer and 183 cases of normal bladder tissues were collected from TCGA,GEO and ArrayExpress public databases to analyze the mRNA expression level of KIF20A,the specimens were sampled during June 2004 and October 2022.Combined with the clinical and pathological features and prognostic data of the TCGA bladder cancer cohort,Chi-square analysis and Kaplan-Meier analysis were used to explore the relationship between KIF20A expression level and the clinicopathological features and prognosis of bladder cancer.Then,the abundance of tumor immune cells was evaluated by TIMER algorithm to explore the relationship between tumor KIF20A expression and immune cell infiltration.Results RNA sequencing samples from 1,145 cases of bladder cancer and 183 cases of normal bladder tissues showed that KIF20A was highly expressed in tumor tissues(P<0.05),with a standard mean difference(SMD)of 1.10.Moreover,the expression level of KIF20A could be distinguished between tumor and normal tissue.However,survival analysis showed no significant association between KIF20A expression and prognosis in patients with bladder cancer.In the exploration of tumor immune microenvironment,it was found that the infiltration levels of dendritic cells,CD8+T cells,neutrophils and macrophages were correlated with the expression of KIF20A.Conclusions KIF20A is over-expressed in bladder cancer tissues,which may be associated with decreased infiltration of immune cells in the tumor microenvironment.KIF20A is a novel potential target for immunotherapy of bladder cancer.