基于环保型样本释放剂联合超声波在病理组织切片制备中的应用价值

Practical Value of Environment-friendly Sample Release Agent Combined with Ultrasound in the Preparation of Pathological Tissue Sections

【目的】探讨环保型样本释放剂联合超声波在病理组织切片制备中的应用价值。【方法】选取2013年2月至2022年12月期间,佛山市中医院送检的2518病理标本为研究对象,同一标本取材两块,随机分成两组,环保快速组:采用环保型样本释放剂联合超声波的快速模式制作病理组织切片;传统组:采用甲醛、乙醇、二甲苯,按照常规的模式制作切片。比较两组切片的苏木素(HE)染色效果、免疫组织化学(IHC)染色效果和非典型性脂肪瘤性肿瘤/高分化脂肪肉瘤(ALT/WDL)组织切片的MDM2基因检测结果的差异。【结果】①两组病理组织的蜡块脱水良好、组织硬度适中。两组切片HE染色后,组织切面完整,无裂隙及颤痕、细胞核与细胞质染色对比恰当、透明度佳、染色均匀、无组织掉片。环保快速组的HE染色优良率和HE染色评分均高于传统组,但是差异均无统计学意义(χ^(2)=3.125,P_(1)=0.070;t=0.965,P_(2)=0.334)。②两组切片IHC染色后,细胞阳性定位准确、呈特异性着色、染色均匀染色强度适中、染色敏感度好、无组织掉片。环保快速组的IHC染色优良率和IHC染色阳性率均低于传统组,但是差异均无统计学意义(χ_(1)^(2)=2.769,P_(1)=0.092;χ_(2)^(2)=0.800,P_(2)=0.375)。③两组WDL组织切片的背景清晰,轮廓分明,染色均匀,细胞清晰可见,细胞核界限清楚,杂交信号在背景荧光下清晰、明亮,无杂信号。两组切片均杂交成功,MDM2均呈阳性扩增。环保快速组杂交成功细胞数低于传统组,但是差异无统计学意义(t=1.414,P=0.230)。【结论】使用环保型样本释放剂联合超声波的组织处理模式,能保障病理组织切片HE染色、IHC染色和MDM2基因检测效果,且更为高效和环保,适合在各级医院中推广使用。

【Objective】To explore the practical value of environment-friendly sample release agent combined with ul⁃trasound in the preparation of pathological tissue sections.【Methods】From February 2013 to December 2022,2518 patho⁃logical specimens submitted by Foshan Municipal Hospital of Traditional Chinese Medicine were selected as the study ob⁃jects.Two samples of the same specimen were randomly divided into two groups:the environment-friendly fast group,in which the pathological tissue sections were made by using the environment-friendly sample release agent combined with ultrasound;and the traditional group,in which formaldehyde,ethanol and xylene were used to make slices in the conven⁃tional way.The differences of hematoxylin(HE)staining effect,immunohistochemistry(IHC)staining effect and MDM2 gene detection result of atypical lipomatous tumor/highly differentiated liposarcoma(ALT/WDL)tissue sections between the two groups were compared【.Results】①The wax of the two groups'pathological tissues was dehydrated well and the tis⁃sue hardness was moderate.After HE staining,the sections of the two groups were intact,without cracks and tremor marks,and the contrast between nucleus and cytoplasm was appropriate,with good transparency,uniform staining,and no tissue loss.The excellent rate and score of HE staining in the environmental fast group were higher than those in the tra⁃ditional group,but the difference was not statistically significant(χ^(2)=3.125,P_(1)=0.070;t=0.965,P_(2)=0.334).②After IHC staining of the two groups of sections,the positive location of the cells was accurate,the staining was specific and uni⁃form,the staining intensity was moderate,the staining sensitivity was good,and there was no tissue loss.The excellent rate of IHC staining and the positive rate of IHC staining in the environmental fast group were lower than those in the tradi⁃tional group,but the difference was not statistically significant(χ_(1)^(2)=2.769,P1=0.092;χ_(2)^(2)=0.800,P2=0.375).③The background and outline of the two groups of WDL tissue sections were clear,the staining was uniform,the cells were clear and visible,the nuclear boundary was clear,the hybridization signal was clear and bright under the background fluores⁃cence,and there was no miscellaneous signal.The two groups of sections were hybridized successfully,and MDM2 showed positive amplification.The number of cells successfully hybridized in the environment-friendly fast group was lower than that in the traditional group,but the difference was not statistically significant(t=1.414,P=0.230).【Conclusions】The tissue treatment method of using environment-friendly sample release agent combined with ultrasound can ensure the de⁃tection effect of HE staining,IHC staining and MDM2 gene detection of pathological tissue sections,and is more efficient and environment-friendly,suitable for promotion and use in hospitals at all levels.