分子印迹技术在分离天然活性成分中的应用研究进展

Research Progress on the Application of Molecular Imprinting Technology in the Separation of Natural Active Ingredients

分子印迹技术因具有特异识别性、高预选性和高稳定性等优点,在天然产物化学领域受到广泛关注,尤其是天然活性物质分离方面。本文对分子印迹技术的基本原理、结合方式、常用原料和聚合方法进行介绍,重点分析了分子印迹聚合物制备方法中的共价作用、非共价作用和金属配位作用等结合方式的区别与联系,同时讨论了2017~2022年分子印迹技术在分离黄酮类、多酚类、生物碱类、有机酸类、甾体类及其他天然活性成分的应用研究进展,并提出了目前分子印迹技术存在的问题和未来研究方向。

Molecular imprinting technology was the process of designing molecularly imprinted polymers against a target molecule as a template,and it had received a lot of attention in the direction of natural product chemistry,especially in the isolation of natural active substances,because of its advantages of specific recognition,high preselection and high stability.In this paper,we introduced the basic principles,binding modes,common raw materials and polymerization methods of molecular imprinting technology,focusing on the different connections of binding modes such as covalent interaction,non-covalent interaction and metal ligand interaction.At the same time,the applications of molecular imprinting technology in the separation of flavonoids,polyphenols,alkaloids,organic acids,steroids and other natural active ingredients in 2017-2022 were also discussed,and the current problems and future research directions of molecular blotting technology were proposed.