《动物研究:体内实验报告》即ARRIVE 2.0指南的解释与阐述(三)

Explanation and Elaboration for the ARRIVE Guidelines 2.0-Reporting Animal Research and In Vivo Experiments(Ⅲ)

提高生物医学研究结果的可重复性是一项重大挑战,研究人员透明且准确地报告其研究过程有利于读者对该研究结果的可靠性进行评估,进而重复该实验或在该成果的基础上进一步探索。ARRIVE 2.0指南是英国国家3Rs中心(NC3Rs)于2019年组织发布的一份适用于任何与活体动物研究报告相关的指导性清单,用以提高动物体内实验设计、实验实施和实验报告的规范性,以及动物实验结果的可靠性、可重复性和临床转化率。ARRIVE 2.0指南的使用不仅可以丰富动物实验研究报告的细节,确保动物实验结果信息被充分评估和利用,还可以使读者准确且清晰地了解作者所表述的内容,促进基础研究评审过程的透明化和完整性。目前,ARRIVE 2.0指南已经被国际生物医学期刊广泛采纳。本文是在国际期刊遵循ARRIVE 2.0指南的最佳实践基础上,对2020年发表于PLoS Biology期刊上的ARRIVE 2.0指南完整解读版(原文请见https://arriveguidelines.org)进行中文编译(第三部分包括“关键10条”里的第8~10条:“实验动物”、“实验步骤”和“结果”部分),以期促进国内研究人员充分理解并使用ARRIVE 2.0指南,提高实验动物研究及报告的规范性,助推我国实验动物科技与比较医学研究的高质量发展。

Improving the reproducibility of biomedical research results is a major challenge.Researchers reporting their research process transparently and accurately can help readers evaluate the reliability of the research results and further explore the experiment by repeating it or building upon its findings.The ARRIVE 2.0 guidelines,released in 2019 by the UK National Centre for the Replacement,Refinement and Reduction of Animals in Research(NC3Rs),provide a checklist applicable to any in vivo animal research report.These guidelines aim to improve the standardization of experimental design,implementation,and reporting,as well as the reliability,repeatability,and clinical translatability of animal experimental results.The use of ARRIVE 2.0 guidelines not only enriches the details of animal experimental research reports,ensuring that information on animal experimental results is fully evaluated and utilized,but also enables readers to understand the content expressed by the author accurately and clearly,promoting the transparency and integrity of the fundamental research review process.At present,the ARRIVE 2.0 guidelines have been widely adopted by international biomedical journals.This article is a Chinese translation based on the best practices of international journals following the ARRIVE 2.0 guidelines in international journals,specifically for the complete interpretation of the ARRIVE 2.0 guidelines published in the PLoS Biology journal in 2020(original text can be found at https://arriveguidelines.org).The third part of the article includes the items 8-10 of ARRIVE 2.0 Essential 10,which covers"experimental animals""experimental procedures"and"results".Its aim is to promote the full understanding and use of the ARRIVE 2.0 guidelines by domestic researchers,enhance the standardization of experimental animal research and reporting,and promote the high-quality development of experimental animal technology and comparative medicine research in China.