雄蚕蛾药材质量标准研究

Study on the Quality Standard of Male Silk Moths

目的:建立雄蚕蛾药材质量控制标准。方法:按照中国药典规定的方法进行薄层鉴别、干燥失重、总灰分、浸出物和蛋白质含量测定研究。结果:以亮氨酸、缬氨酸和丙氨酸和雄蚕蛾对照药材鉴别氨基酸类成分;干燥失重介于2.07%~3.11%;总灰分介于3.73%~5.19%;醇溶性浸出物介于10.98%~15.44%;可溶性蛋白含量的线性相关系数为0.996;精密度RSD为1.48%;重复性RSD为1.28%,120 min内稳定性良好,RSD为1.69%;准确度平均回收率为99.21%,RSD为1.75%,10批雄蚕蛾药材蛋白质含量介于2.58%~3.76%。结论:本研究建立的雄蚕蛾药材质量控制方法简单、稳定、可靠,成本可控。

Objective:To establish the quality control standard of male silk moths.Methods:According to the provisions of the Chinese pharmacopoeia methods for dry weight loss,TLC,total ash,extract determination and content determination.Results:Leucine,valine and alanine were used as control materials to identify amino acid components from male silk moths.The dry weight loss content ranged 2.07%~3.11%;the total ash content ranged 3.73%~5.19%;the ethanol-soluble extractives content ranged 10.98%~15.44%.The content of soluble protein in male silkworm moth was determined by UV-VIS spectrophotometry,the linear correlation coefficient was 0.996;RSD of precision test was 1.48%;RSD of repeatability test was 1.28%;the stability was good within 120 min,RSD was 1.69%;The average recovery was 99.21%and RSD was 1.75%;The total protein content of 10 batches of male silkworm moth was 2.58%~3.76%.Conclusion:The quality control method of male silkworm moth established in this study is simple,stable,reliable and cost controllable.