丹霞梧桐组织培养技术研究

Study on Tissue Culture and Propagation of Firmiana danxiaensis Seeds

采用丹霞梧桐种子和苗木嫩茎为外植体,研究不同外植体类型、不同灭菌方式、不同培养基对丹霞梧桐组织培养的影响,结果表明,种子用0.1%的升汞处理8 min或15%的次氯酸钠处理8 min,两种消毒方式升汞消毒效果最佳。顶芽、嫩茎用0.1%的升汞分三次(4 min+3 min+1 min),每次间隔用无菌水冲洗1次消毒效果最佳。初代培养基配方:MS+6-BA1.0 mg/L+NAA0.5 mg/L+蔗糖30 g/L+琼脂5 g/L.MS+6-BA0.5 mg/L+NAA0.5 mg/L+糖30 g/L+活性炭0.5 g/L+琼脂5 g/L诱导发芽率达90~95%、继代培养基:MS+6-BA1.0 mg/L+GA30.5 g/L+蔗糖30 g/L+琼脂5 g/L,增值倍数为3~6。

In this study,seeds and tender stems of Firmiana danxiaensis were used as explants to study the effects of different explant types,different sterilization methods and different media on tissue culture of Firmiana danxiaensis,laying a foundation for artificial breeding of Firmiana danxiaensis seedlings.The experiment showed that the seeds were treated with 0.1%Hg for 8min or 15%Sodium hypochlorite for 8min,the two disinfection methods of Hg for 8min had the best disinfection effect.The tender stem of the terminal bud was rinsed with 0.1%Hg for three times(4 min+3 min+1 min),and sterile water was used for one time at each interval for the best disinfection effect.The initial medium formula:MS+6-BA1.0 mg/L+NAA0.5 mg/L+sucrose 30 g/L+AGAR 5 g/L+6-BA0.5 mg/L+NAA0.5 mg/L+sugar 30 g/L+activated carbon 0.5 g/L+AGAR 5 g/L to induce germination rate up to 90~95%.The subculture medium:MS+6-BA1.0 mg/L+GA30.5 g/L+sucrose 30 g/L+AGAR 5 g/L,the multiplication ratio was 3-6.