锯缘青蟹产组胺菌的分离与鉴定

Isolation and Identification of Histamine-Producing Bacteria from Scylla serrata

本研究从锯缘青蟹中分离得到24株产组胺菌。经检测,24株产组胺菌均携带组氨酸脱羧酶hdc基因,通过16S rDNA比对分析,共鉴定为9个属15个细菌种,其中Acinetobacter baumannii为优势分离菌种,占比为25.0%;产组胺能力检测表明,24株产组胺菌发酵上清液平均组胺含量为33.97 mg·L^(-1),Aeromonas hydrophilaH_(20)的组胺含量最高达45.06 mg·L^(-1)。本研究为进一步了解锯缘青蟹产组胺微生物种类,以及加强锯缘青蟹产品质量安全控制提供一定参考。

In this study,24 strains of histamine producing bacteria were isolated from Scylla serrata.All these 24 bacterial strains carried the histidine decarboxylase gene hdc.A total of 9 genera and 15 bacterial species were identified through 16S rDNA analysis,among which Acinetobacter baumannii was the dominant isolated strain,accounting for 25.0%.The average histamine content in the fermentation supernatant of the 24 strains was 33.97 mg·L^(-1),with the highest histamine content 45.06 mg·L^(-1)in Aeromonas hydrophilaH_(20).This study provides some reference for further comprehension of the types of histamine producing microorganisms and the quality and safety control in Scylla serrata.