HPLC-UV法同时测定胡萝卜中5种有机酸的含量

Simultaneous Determination of 5 Organic Acid in Carrot by HPLC-UV

本研究建立了高效液相色谱-紫外检测器同时测定胡萝卜中5种有机酸的方法,其在各自的范围内线性关系良好,检出限范围为0.0158~0.0607 g·kg^(-1),定量限范围为0.0526~0.2024 g·kg^(-1),回收率范围为85%~115%,RSD范围为0.46%~3.20%。胡萝卜样品中均含有5种有机酸,该方法的准确性较好,可用于胡萝卜中有机酸的测定。

In this study,high performance liquid chromatography-ultraviolet absorption detector method was established for the simultaneous determination of five organic acids in carrot.The linear relationships were good in their respective ranges.The detection limits ranged from 0.0158 g·kg^(-1)to 0.0607 g·kg^(-1),The limits of quantification ranged from 0.0526 g·kg^(-1)to 0.2024 g·kg^(-1),the recovery rates ranged from 85%to 115%,RSD ranged from 0.46%to 3.20%.Carrot samples all contain five organic acids,the method is accurate and can be used for the determination of organic acids in carrot.