病原哈维氏弧菌(Vibrio harveyi)TS-628菌株基因组分析

Genome analysis of pathogenic Vibrio harveyi strain TS-628

哈维氏弧菌是海洋鱼类和无脊椎动物的重要条件致病菌,经常造成海水养殖动物严重病害,给水产养殖业造成巨大的经济损失,而不同来源的哈维氏弧菌的毒力存在很大差异。本研究采用第二代Illumina Hiseq平台与第三代PacBio平台相结合的测序技术,对分离自福建海域患病青石斑鱼的哈维氏弧菌TS-628菌株进行基因组测序和分析,挖掘该菌的基因组信息,从基因组层面了解该病原菌的毒力相关基因,以期为哈维氏弧菌后续致病机制的研究以及哈维氏弧菌病防治提供有力数据支撑。结果表明,哈维氏弧菌TS-628菌株基因组含2条染色体、2个质粒,其中1个质粒为新发现质粒;基因组大小为6151198 bp,基因组GC含量为44.68%,共编码5658个基因,共预测到毒力基因747个,毒力基因主要与细菌的黏附、摄铁系统及分泌系统有关;此外,还发现耐药基因294个,反映该菌株不仅具有编码多种毒力因子的潜力,同时具有抗多种药物的可能。

V.harveyi is an important opportunistic pathogen of marine fish and invertebrates,which often causes serious diseases of mariculture animals and brings huge economic losses to the aquaculture industry.However,the virulence of V.harveyi isolated from different environments varies greatly.In the present study,the sequencing technology combining the second generation Illumina Hiseq platform and the third generation PacBio platform was used to sequence and analyze the genome of V.harveyi strain TS-628 isolated from the diseased Epinephelus awoara.The genome information of this strain was exploited to understand the virulence related genes of the pathogen,in order to provide data support for the subsequent research on the pathogenesis of V.harveyi and control of V.harveyi disease.The results revealed that the genome of V.harveyi strain TS-628 had two chromosomes and two plasmids.One of the plasmids was a newly discovered plasmid.The genome size of this strain was 6151198 bp,the GC content of the genome was 44.68%,the number of predicted genes were 5658,the number of predicted virulence genes were 747.The 747 virulence genes were mainly related to bacterial adhesion,iron uptake and secretion systems.In addition,294 drug-resistant genes were found,reflecting that the V.harveyi strain TS-628 not only had the potential to encode multiple virulence factors,but also had the potential to resist multiple antibacterial agents.