摘要
长链非编码RNA(lncRNA)的亚细胞定位和其功能息息相关,定位于细胞核时,lncRNA可以维持染色质结构,调控基因转录,参与mRNA的可变剪接;定位于细胞质时参与信号传导、转录后调控、翻译和翻译后修饰;定位于细胞器时协助完成细胞器的功能。lncRNA的亚细胞定位机制与其自身序列、结合蛋白等密切相关。此外通过构建核滞溜载体Snovector、添加胞质定位元件等强制改变lncRNA的亚细胞定位,以及利用APEX2等技术研究lncRNA亚细胞定位和其功能之间的关系,有利于lncRNA疗法的开发。
The subcellular localization of long non-coding RNA(lncRNA)is closely related to its function.When localized in the nucleus,lncRNA can maintain chromatin structure,regulate gene transcription and participate in variable splicing of mRNA.When localized in the cytoplasm,lncRNA can mediate signal transduction,post-transcriptional regulation,translation and post-translational modification.When localized in the organelles,lncRNA can assist in the function of them.The subcellular localization of lncRNA is closely related to its own sequences and binding proteins.In addition,the development of lncRNA therapy will be facilitated by changing lncRNA subcellular localization in use of constructing Snovector,adding cytosolic localization elements,or techniques like APEX2,which indicates the relationship between the subcellular localization of lncRNA and its function.
作者
苏越
梁琳慧
何祥火
SU Yue;LIANG Linhui;HE Xianghuo(Institutes of Biomedical Sciences,Fudan University,Shanghai 200032;Cancer Institute,Fudan University Shanghai Cancer Center,Shanghai 200032,China)
出处
《基础医学与临床》
2023年第10期1580-1584,共5页
Basic and Clinical Medicine
基金
国家自然科学基金(81972253)。
