CD14和IL-2R基因多态性与脑出血术后颅内感染的关联

Association of CD14,IL-2Rgene polymorphisms with postoperative intracranial infection in cerebral hemorrhage patients

目的探讨脂多糖受体CD14和白细胞介素-2受体(IL-2R)基因多态性与脑出血术后颅内感染的关联。方法选取2018年3月-2020年12月在河南省人民医院行脑出血手术的患者162例为研究对象,根据患者术后是否发生颅内感染分为感染组28例和未感染组134例,分析感染组病原菌,比较两组临床资料,检测CD14、IL-2R基因多态性。结果28例感染患者共分离病原菌36株,其中革兰阳性菌21株(58.33%)、革兰阴性菌13株(36.11%)、真菌2株(5.56%);感染组患者CD14 rs2569190位点TT基因、T等位基因频率高于未感染组(P<0.05);感染组患者IL-2R rs743777位点GG基因频率高于未感染组(P<0.05);Logistic回归分析显示,术后再出血、有脑脊液漏、CD14 rs2569190位点TT基因型(P<0.05)均为影响脑出血术后颅内感染的危险因素(P<0.05)。结论CD14、IL-2R基因多态性与脑出血术后颅内感染相关,术后再出血、有脑脊液漏、CD14 rs2569190位点TT型是脑出血术后颅内感染的危险因素。

OBJECTIVE To explore the association of lipopolysaccharide receptor(CD14),interleukin-2receptor(IL-2R)gene polymorphisms with postoperative intracranial infection in cerebral hemorrhage patients.METHODS A total of 162cerebral hemorrhage patients who received surgical procedures in Henan People′s Hospital from Mar 2018to Dec 2020were recruited as the research subjects and were divided into the infection group with 28cases and the no infection group with 134cases according to the status of postoperative intracranial infection.The clinical data were compared between the two groups,and the CD14and IL-2Rgene polymorphisms were detected.RESULTS Totally 36strains of pathogens were isolated from the 28patients with infection,21(58.33%)of which were gram-positive bacteria,13(36.11%)were gram-negative bacteria,and 2(5.56%)were fungi.The frequencies of TT genotype and T allele at CD14rs2569190locus were significantly higher in the infection group than in the no infection group(P<0.05);the frequency of GG genotype at IL-2Rrs743777locus was significantly higher in the infection group than in the no infection group(P<0.05).Logistic regression analysis showed that postoperative recurrent hemorrhage,leakage of cerebrospinal fluid and TT genotype at CD14rs2569190locus were the risk factors for the postoperative intracranial infection in the cerebral hemorrhage patients(P<0.05).CONCLUSION The CD14and IL-2Rgene polymorphisms are associated with the postoperative intracranial infection in the cerebral hemorrhage patients.The postoperative recurrent hemorrhage,leakage of cerebrospinal fluid and TT genotype at CD14rs2569190locus are the risk factors for the postoperative intracranial infection.