CCND1基因多态性及幽门螺杆菌感染与胃癌易感性的关联

Association of CCND1 gene polymorphisms,Helicobacter pylori infection with susceptibility to gastric cancer

目的分析细胞周期蛋白D1(CCND1)基因多态性及幽门螺杆菌(Hp)感染与胃癌易感性的关联。方法选择郑州市第七人民医院2017年1月-2019年1月收治的82例胃癌患者为胃癌组和70例慢性胃炎患者为慢性胃炎组,采用聚合酶链反应-限制性片段长度多态性分析和产物直接测序技术检测CCND1基因单核苷酸多态性位点多态性。结果胃癌组Hp感染阳性率(67.07%)和慢性胃炎组(60.00%)比较,无统计学差异。两组CCND1基因rs9344位点AA、AG、GG基因型及A、G等位基因频率比较,差异均有统计学意义(P<0.05)。经Logistic回归分析发现,Hp感染阳性患者中,两组CCND1基因rs9344位点AA、AG、GG基因型及A、G等位基因频率比较,差异均有统计学意义(P<0.05);Hp感染阴性患者中,两组CCND1基因rs9344位点AA、AG、GG基因型及A、G等位基因频率比较,均无统计学差异。Hp感染阳性患者中,在隐性遗传模式下,两组CCND1基因rs9344位点AA和AG+GG基因型频率比较,差异有统计学意义(P<0.05)。Hp感染阳性AA基因型和Hp感染阳性AG+GG基因型胃癌患者3年生存率比较,差异有统计学意义(P<0.05)。结论Hp感染及CCND1基因rs9344位点AA基因型与胃癌易感性及预后密切相关,Hp感染阳性AA基因型携带者胃癌易感性增加,术后生存时间明显缩短。

OBJECTIVE To observe the association of cyclin D1(CCND1)gene polymorphism,Helicobacter pylori(Hp)infection with susceptibility to gastric cancer.METHODS Totally 82 gastric cancer patients and 70 chronic gastritis patients who were treated in the Seventh People's Hospital of Zhengzhou from Jan 2017 to Jan 2019 were respectively assigned as the gastric cancer group and the chronic gastritis group.The single nucleotide polymorphisms of CCND1 gene were detected by polymerase chain reaction-restriction fragment length polymorphism assay and direct sequencing for products.RESULTS The positive rate of Hp infection was 67.07%in the gastric cancer group,60.00%in the chronic gastritis group,and there weas no significant difference.There were significant differences in the frequencies of AA,AG,GG genotypes and A,G alleles at rs9344 locus of CCNDl gene between the two groups(P<o.05).Logistic regression analysis showed that among the patients who were positive for Hp infection,there were significant differences in the frequencies of AA,AG,GG genotypes and A,G alleles at rs9344 locus of CCND1 gene between the two groups(P<0.05);among the patients who were negative for Hp infection,there were no significant differences in the frequencies of AA,AG,GG genotypes and A,G alleles at rs9344 locus of CCNDl gene between the two groups.Among the patients who were positive for Hp infection,there were significant differences in the frequencies of AA and AG plus GG genotypes at rs9344 locus of CCND1 gene between the two groups under recessive genetic model(P<0.05).There was significant difference in the 3-year survival rate between the positive Hp infection patients with AA genotype and the positive Hp infection patients with AA plus GG genotype(P<0.05).CONCLUSION The Hp infection and AA genotype at rs9344 locus of CCNDl gene are closely associated with the susceptibility to gastric cancer and prognosis.The positive Hp infection patients carrying with AA genotype have the increased susceptibility to gastric cancer,and the postoperative survival time is remarkably shortened.