JNK1基因多态性与中耳炎听力损失的关联

Association of JNK1 gene polymorphisms with hearing loss patients with otitis media

目的探讨C-JUN n末端激酶1(JNK1)基因多态性与中耳炎听力的关联。方法选择武汉理工大学医院耳鼻喉科2018年1月-2021年5月因感染所致中耳炎入院接受治疗患者66例为研究对象,根据听力计检测患者听阈中位数,分为听力损失较轻组33例和听力损失较重组33例。收集两组患者性别、年龄、体质量指数、发病部位、合并耳鸣和合并眩晕情况,检测所有患者气导听阈、骨导听阈、气骨导差、咽鼓管功能不良、鼓室病变和听骨链病变情况,采用聚合酶链式扩增反应(PCR)扩增目的基因序列片段。结果听力损失较重组Rs9284位点携带GG基因型和G等位基因频率高于听力损失较轻组(P<0.05),两组rs9284位点TT、TG基因型、Rs11598320位点AA、AT和TT基因型和A/T等位基因频率比较,无统计学差异;Rs9284位点携带GG型患者气导听阈高于TT/TG型,气骨导差低于TT/TG型(P<0.05);两组咽鼓管功能不良、鼓室病变和听骨链病变比较,无统计学差异。结论JNK1基因rs9284位GG携带者感染所致听力损失较为严重,听力学表现较差,筛查该位点对于指导临床干预有一定意义。

OBJECTIVE To explore the association of C-JUN N-terminal kinases(JNKs)gene polymorphisms with severity of hearing loss of the patients with otitis media.METHODS A total of 66 patients who were treated in department of otolaryngology of Wuhan University of Science and Technology Hospital due to infection-induced otitis media from Jan 2018 to May 2021 were recruited as the research subjects and were divided into the mild hearing loss group with 33 cases and the severe hearing loss group with 33 cases according to the median hearing threshold measured by audiometer.The gender,age,body mass index,pathogenic site and incidence of complications with tinnitus and dizziness were collected from the two groups of patients.The air conduction hearing threshold,bone conduction hearing threshold,air bone gap,eustachian tube dysfunction,tympanic lesions and ossicular chain lesions were detected for all of the patients.The sequence fragments of target genes were amplified by polymerase chain reaction(PCR).RESULTS The frequencies of GG genotype and G allele at Rs9284 locus were significantly higher in the severe hearing loss group than in the mild hearing loss group(P<0.05);there were no significant differences in the frequencies of TT and TG genotypes at rs9284 locus and AA,AT,TT genotypes and A/T alleles at Rsl1598320 locus between the two groups.The air conduction hearing threshold of the patients carrying with GG genotype at Rs9284 locus was higher than that of the patients carrying with TT/TG genotype,while the air bone gap of the patients carrying with GG genotype was significantly lower than that of the patients carrying with TT/TG genotype(P<0.05).There were no significant differences in the eustachian tube dysfunction,tympanic lesions and ossicular chain lesions between the two groups.CONCLUSION The patients who carry with GG genotype at rs9284 locus of JNK1 gene have more severe infection-induced healing loss and poorer audiological performance,and the screening of the locus may have certain significance for guiding the clinical intervention.