儿童轮状病毒感染性肠炎外周血JAK2/STAT3信号通路表达及意义

Expressions of JAK2/STAT3 signaling pathways in peripheral blood of children with rotavirus infectious enteritis and their clinical significance

目的探讨儿童轮状病毒感染性肠炎外周血1,25-二羟维生素D_(3)[1,25-(OH)_(2)VitD_(3)]、外周血CD11b、JANUS激酶2(JAK2)/信号转导和转录激活因子3(STAT3)信号通路表达及临床意义。方法选取2020年1月-2021年1月重庆市江津区妇幼保健院儿内科收治的127例轮状病毒感染性肠炎患儿为病例组,随机选取132名同期健康体检儿童为对照组,检测两组血清1,25-(OH)_(2)VitD_(3)、C-反应蛋白(CRP)、降钙素原(PCT)、免疫球蛋白(Ig)M、IgA及外周血CD11b、JAK2 mRNA、STAT3 mRNA、CD_(4)^(+)/CD_(8)^(+)水平,并分析上述指标间的相关性。结果病例组大便频率、体温、外周血CD11b、JAK2 mRNA、STAT3 mRNA及血清CRP、PCT高于对照组(P<0.05);血清1,25-(OH)_(2)VitD_(3)、IgM、IgA及外周血CD_(4)^(+)/CD_(8)^(+)低于对照组(P<0.05)。病例组患儿按病情严重程度分组后结果显示,重度组血清1,25-(OH)_(2)VitD_(3)水平低于中度组和轻度组,中度组低于轻度组(P<0.05);重度组患儿外周血CD11b、JAK2 mRNA、STAT3 mRNA水平高于中度组和轻度组,中度组高于轻度组(P<0.05)。上述指标间均具有相关性(P<0.05)。结论轮状病毒感染性肠炎患儿血清1,25-(OH)_(2)VitD_(3)呈低表达,且其随病情加重而递减,而外周血CD11b、JAK2 mRNA、STAT3 mRNA呈高表达,且其随病情加重而递增,四指标可反映炎症病变进程及免疫功能。

OBJECTTVE To explore the expressions of 1,25-Dihydroxyvitamin D_(3),(1,25-(OH)_(2) VitD_(3)),CD11b,JANUS kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)signaling pathways in peripheral blood of children with rotavirus infectious enteritis and analyze the clinical significance.METHODS A total of 127 children with rotavirus infectious enteritis who were treated in Jiangjin Maternal and Child Health Hospital from Jan 2020 to Jan 2021 were assigned as the case group,meanwhile,132 healthy children who received physical examination were chosen as the control group.The levels of serum 1,25-(OH)_(2)VitDs.C-reactive proteome(CRP),procalcitonin(PCT),immunoglobulin(Ig)M,IgA,peripheral blood CD11b.JAK2 mRNA,STAT3 mRNA and CD_(4)^(+)/CD_(8)^(+)were detected for the two groups of research subjects,and the correlation between the above indexes was observed.RESULTS The frequency of defecation,body temperature,peripheral blood CD1lb,JAK2 mRNA,STAT3 mRNA,serum CRP and PCT were higher in the case group than in the control group(P<0.05);the serum 1,25-(OH)_(2)VitD_(3),IgM,IgA and peripheral blood CD_(4)^(+)/CD_(8)^(+)were lower in the case group than in the control group(P<o.05).The children of the case group were grouped according to llness condition,the serum 1,25-(OH)_(2)VitD,level of the severe group was lower than that of the moderate group and the mild group,and the serum 1,25-(OH),VitD,level of the moderate group was lower than that of the mild group(P<0.05);the expression levels of peripheral blood CD11b,JAK2 mRNA and STAT3 mRNA of the severe group were higher than those of the moderate group and the mild group,and the expression level of above indexes of the moderate group were higher than those of the mild group(P<0.05).There was correlation between the above indexes(P<0.05).CONCLUSION The children with rotavirus infectious enteritis show low expression level of serum 1,25-(OH)_(2)VitDs,which declines with the aggravation of illness condition;the children show high expression levels of peripheral blood CD11b,JAK2 mRNA and STAT3 mRNA,which are elevated with the aggravation of illness condition.The four indexes may reflect the progression of inflammatory lesions and immune function.