款冬花水提物对肥胖小鼠糖脂代谢的影响

Effects of Water Extracts from Farfarae Flos on Glucose and Lipid Metabolism in Obese Mice

目的研究款冬花水提物对肥胖小鼠血脂血糖的调节作用。方法采用化学方法对款冬花水提物成分进行鉴定;用紫外可见分光光度法测定款冬花水提物中总萜的含量;将小鼠随机分为空白组,模型组,奥利司他组以及款冬花水提物低、中、高剂量组,空白组给予普通饲料喂养,其余各组采用高脂高糖饲料喂养制备肥胖动物模型,边造模边给药45 d。实验结束后,检测血清中甘油三酯(triglyceride,TG)、胆固醇(cholesterol,CHO)、高密度脂蛋白胆固醇(highdensity lipoprotein-C,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein-C,LDL-C)、葡萄糖(glucose,GLU)、糖化血清蛋白(glycosylatedserum protein,GSP)含量,采用苏木素-伊红(HE)和油红O染色观察小鼠肝脏组织形态变化和脂滴分布。结果款冬花水提物含有黄酮类和萜类化合物;与空白组相比,模型组TG、CHO、LDL-C、GLU、GSP水平显著升高(P<0.01或P<0.05),HDL-C显著降低(P<0.05),肝脏中脂滴聚集面积显著增加(P<0.01);与模型组相比,款冬花水提组均能显著降低TG、LDL-C、CHO、GLU、GSP,升高HDL-C,减少脂滴的蓄积,给药各组血脂血糖状况均较模型组显著降低,其中款冬花水提物高剂量组效果最好。结论款冬花水提物能够通过降低血脂、CHO,改善肝脏组织病理形态,起到改善肥胖小鼠糖脂代谢的作用。

OBJECTIVE To study the moderation effect of water extract of Farfarae Flos on blood lipid and glucose regulation in obese mice.METHODS Chemical method was used to identify the components of the water extract of Farfarae Flos.The contents of total terpenes in the water extract of the Farfarae Flos were determined by UV-VIS spectrophotometry.The mice were randomly divided into blank group,model group,orlistat group,and low-dose,medium-dose,and high-dose groups of water extract of Farfarae Flos.The blank group was fed with ordinary diet,while the other groups were fed with high-fat and high-sugar diet to establish an obese animal model,and they were administration while molding for 45 d.At the end of the experiment,the contents of serum triglyceride(TG),cholesterol(CHO),high-density lipoprotein-C(HDL-C),low-density lipoprotein-C(LDL-C),glucose(GLU),and glycosylated serum protein(GSP)were detected.Hematoxylin-eosin(HE)and oil red O staining were used to observe the morphological changes and lipid droplet distribution of liver tissue in mice.RESULTS There were flavonoids and terpenoids in the water extract of the flower.Compared with the blank group,the levels of TG,CHO,LDL-C,GLU and GSP in the model group were significantly increased(P<0.05 or P<0.01),HDL-C was significantly decreased(P<0.05),and the area of lipid droplets in the liver was significantly increased(P<0.01).Compared with the model group,TG,LDL-C,CHO,GLU and GSP levels in the Farfarae Flos water extract groups were significantly decreased,HDL-C was increased,and lipid droplet accumulation were reduced.Compared with the model group,the blood lipid and blood glucose levels in the treatment groups were significantly decreased,among them,the high-dose group of the water extract of Farfarae Flos had the best effect.CONCLUSION The water extract of farfara can improve glucose and lipid metabolism in obese mice by reducing blood lipids,CHO,and improving the pathological morphology of liver tissue.