肠道菌Bombella intestini对意大利蜜蜂营养代谢的影响

Effects of the enterobacteria Bombella intestini on the nutritional metabolism of Apis mellifera

【目的】探究肠道菌Bombella intestini对意大利蜜蜂Apis mellifera营养代谢的影响。【方法】采用无菌工蜂模型构建的方法培养无菌新蜂300只,随机分为2个组,每个组3个重复,每个重复50只。对照组(CK)饲喂40%无菌蔗糖水,试验组(BI)饲喂含106cfu/mLB.intestini的40%蔗糖水,两组均饲喂无菌花粉料与无菌去离子水。每日按时饲喂并记录采糖量、采粉量、蜂体重。于第9天采集样品测定身体成分、血淋巴生化指标、营养代谢相关基因表达等。【结果】BI组平均日采糖量显著高于CK组(P<0.05),但是蜂体重并无显著差异(P>0.05);身体成分分析表明,BI组干物质、粗脂肪含量均显著低于CK组(P<0.05),粗灰分含量显著高于CK组(P<0.05),粗蛋白含量略高于CK组,碳水化合物略低于CK组,统计学差异不显著(P>0.05);BI组的血淋巴中总蛋白(TP)、甘油三酯(TG)、胆固醇(TCHO)和高密度脂蛋白(HDL)含量均显著低于CK组(P<0.05);BI组细胞色素B(CytB)、细胞色素C(CytC)、卵黄原蛋白(Vg)、胰岛素肽类(ILP)和胰岛素受体(LnR)均显著上调;其中CytB和CytC是参与线粒体生物氧化的两种线粒体蛋白,其表达量上调说明物质分解代谢水平提高。【结论】肠道菌B.intestini可以改善蜜蜂食欲,促进采食,增加蜂体营养储备,促进养分的分解代谢。

[Objectives]To investigate the effects of the enterobacterium Bombella intestini on the nutritional metabolism of Apis mellifera.[Methods]A total of 300 aseptic new bees were cultured using the aseptic worker bee model and randomly divided into 2 groups with 3 replicates per group and 50 new bees per replicate.The control group(CK)was fed 40%sterile sucrose solution,and the experimental group(BI)was fed 40%sucrose solution containing 106 cfu/ml of B.intestini.Both groups were also fed sterile pollen and sterile deionized water.The bees were fed every day and their body weight,and the amount of sugar and pollen consumed,were recorded.On day 9,individual bees were sampled to determine their body composition,hemolymph biochemical indexes and the expression of genes related to nutrient metabolism.[Results]The results showed that the average daily sugar intake of the BI group was significantly higher than that of the CK group(P<0.05),however,there was no significant difference in the body weight of the two groups(P>0.05).The dry matter and lipid content of the BI group were significantly lower than those of the CK group(P<0.05).However,the ash content of the BI group was significantly higher than that of the CK group(P<0.05).The crude protein content of the BI group was slightly higher than that in CK group,whereas its carbohydrate content was slightly lower;both these differences were not statistically significant.