黑色素瘤相关抗原3、zeste 2多梳抑制复合物2亚基增强子在宫颈癌组织中的表达及与患者预后的关系

Expression of melanoma-associated antigen 3,enhancer of zeste 2 polycomb repressive complex 2 subunit in cervical cancer tissues and their relationship with patients' prognosis

目的 探讨黑色素瘤相关抗原3(MAGEA3)、zeste 2多梳抑制复合物2亚基增强子(EZH2)在宫颈癌组织中的表达及与患者预后的关系。方法 选取45例宫颈癌患者的宫颈癌组织、18例宫颈上皮内瘤变(CIN)患者的CIN组织和17例健康体检者的正常宫颈组织,采用免疫组化法检测MAGEA3、EZH2的表达情况。MAGEA3、EZH2表达的相关性采用Spearman相关分析;宫颈癌患者预后的影响因素采用多因素Logistic回归分析。结果 宫颈癌组织中MAGEA3、EZH2的阳性表达率均高于正常宫颈组织和CIN组织,差异均有统计学意义(P﹤0.05)。淋巴结转移、肌层浸润深度﹥1/2、临床分期为ⅡA期、组织学分级为G3级宫颈癌患者宫颈癌组织中MAGEA3、EZH2的阳性表达率分别高于无淋巴结转移、肌层浸润深度≤1/2、临床分期为Ⅰ期、组织学分级为G1~2级的患者,差异均有统计学意义(P﹤0.05)。宫颈癌组织中MAGEA3的表达与EZH2的表达呈正相关(r=0.337,P﹤0.05)。MAGEA3、EZH2阳性表达宫颈癌患者的3年生存率均高于阴性表达患者,差异均有统计学意义(P﹤0.05)。多因素Logistic回归分析结果显示,淋巴结转移、肌层浸润深度﹥1/2、临床分期为ⅡA期、组织学分级为G3级均是宫颈癌患者预后的独立危险因素(P﹤0.05)。结论 MAGEA3、EZH2在宫颈癌组织中表达上调,且阳性表达患者预后较差,淋巴结转移、肌层浸润深度﹥1/2、临床分期为ⅡA期、组织学分级为G3级均是宫颈癌患者预后的独立危险因素。

Objective To investigate the expression of melanoma-associated antigen 3(MAGEA3),enhancer of zeste 2 polycomb repressive complex 2 subunit(EZH2)in cervical cancer tissues and their relationship with patients’progno-sis.Method The cervical cancer tissues from 45 cervical cancer patients,cervical intraepithelial neoplasia(CIN)tissues from 18 CIN patients,and normal cervical tissues from 17 healthy individuals were selected,immunohistochemistry was used to detect the expression of MAGEA3 and EZH2.The correlation between the expression of MAGEA3 and EZH2 was analyzed by Spearman correlation.The prognostic factors of cervical cancer patients were analyzed using multivari-ate Logistic regression.Result The positive expression rates of MAGEA3 and EZH2 in cervical cancer tissues were higher than those in normal cervical tissues and CIN tissues,and the differences were statistically significant(P<0.05).The positive expression rates of MAGEA3 and EZH2 in cervical cancer tissues of patients with lymph node metastasis,depth of myometrial invasion>1/2,clinical stage IIA,and histological grade G3 were higher than those in patients without lymph node metastasis,depth of myometrial invasion≤1/2,clinical stage I,and histological grade G1-2,and the differenc-es were statistically significant(P<0.05).The expression of MAGEA3 in cervical cancer tissues was positively correlated with that of EZH2(r=0.337,P<0.05).The 3-year survival rates of cervical cancer patients with MAGEA3 and EZH2 posi-tive expression were higher than those of patients with negative expression,and the differences were statistically signifi-cant(P<0.05).Multivariate Logistic regression analysis showed that lymph node metastasis,depth of myometrial inva-sion>1/2,clinical stage IIA,and histological grade G3 were independent risk factors for the prognosis of patients with cer-vical cancer(P<0.05).Conclusion MAGEA3 and EZH2 are up-regulation in cervical cancer tissues,and patients with positive expression tend to endure a poor prognosis.Lymph node metastasis,depth of myometrial invasion>1/2,clinical stage IIA,and histological grade G3 are independent risk factors for the prognosis of cervical cancer patients.