糖异平对糖耐量减低合并非酒精性脂肪肝大鼠氧化应激及炎症因子表达的影响

Effect of Tangyiping on oxidative stress and expression of inflammatory factors in rats with impaired glucose tolerance combined with non-alcoholic fatty liver disease

目的 观察糖异平对糖耐量减低(IGT)合并非酒精性脂肪肝(NAFLD)大鼠氧化应激及炎症因子表达的影响。方法 将40只大鼠随机分为对照组(n=10)以及实验组(n=30)。对照组给予正常饮食,实验组通过高糖、高脂饮食培养IGT合并NAFLD模型的大鼠,8周后选取20只实验组模成功大鼠根据随机分配原则分为成模型组(n=10)及治疗组(n=10)。对照组及模型型组均给予生理盐水灌胃,治疗组给予中药糖异平灌胃。药物干预4周后,比较各组大鼠血清中血糖水平[空腹血糖(FBG)、餐后2 h血糖(2 hPG)]、血脂水平[三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)]、炎症因子水平[白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)和白细胞介素1β(IL-1β)]以及氧化应激指标[丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)]。取出大鼠肝脏称重后再行HE染色,光镜下观察大鼠的肝脏组织病理情况。结果 与模型组相比,糖异平治疗组可以有效降低大鼠血糖、血脂及炎症因子水平,并改善氧化应激反应,差异有统计学意义(P <0.05)。结论 糖异平可能通过下调大鼠炎症因子IL-6、TNF-α以及IL-1β的表达,来改善氧化应激反应,从而对IGT合并NAFLD起到治疗作用。

Objective To observe the effect of Tangyiping on oxidative stress and expression of inflammatory factors in rats with impaired glucose tolerance(IGT)combined with non-alcoholic fatty liver disease(NAFLD).Methods A total of 40 rats were randomly divided into a control group(n=10)and an experimental group(n=30).The rats in the control group had a normal diet,while the model rats with IGT combined with NAFLD in the experimental group were fed a high-sugar and high-fat diet.After 8 weeks,20 rats in the experimental group with successful membrane formation were randomly divided into a model group(n=10)and a treatment group(n=10)according to the principle of random allocation.The control group and the model group were given physiological saline by gavage,while the treatment group was given traditional Chinese medicine Tangyiping by gavage.After 4 weeks of drug intervention,the levels of blood glucose(fasting blood glucose[FBG],2 h postprandial blood glucose[2 hPG]),blood lipid levels(triglyceride[TG],total cholesterol[TC],low-density lipoprotein[LDL],high-density lipoprotein[HDL]),levels of inflammatory factors(interleukin-6[IL-6],tumor necrosis factor-α[TNF-α],interleukin-1β[IL-1β])and oxidative stress indicators[malondialdehyde[MDA],superoxide dismutase[SOD],glutathione[GSH])were compared.The liver was taken out and weighed before HE staining.The histopathology of the liver was observed under a light microscope.Results Compared with the model group,the levels of blood sugar,blood lipid,and inflammatory factors of rats in the group treated with Tangyiping were effectively reduced,and oxidative stress response was improved,with statistically significant differences(P<0.05).Conclusion Tangyiping may downregulate the inflammatory factors IL-6,TNF-α,and IL-1βin rats to improve oxidative stress response and thus play a therapeutic role against the development of IGT combined with NAFLD.