多伞阿魏抗胃癌活性部位的急性毒性试验研究

Acute toxicity of anti-gastric cancer active ingredients of Ferula ferulaeoides

目的研究多伞阿魏抗胃癌活性部位的急性毒性,初步评价多伞阿魏抗胃癌活性部位的安全性,为其抗胃癌药物研发与临床用药提供参考。方法采用最大给药量法给小鼠灌胃多伞阿魏抗胃癌活性部位混悬液;给药前和给药后第14天分别称量小鼠体重;连续14 d观察小鼠的一般状况和死亡情况;给药后第14天小鼠眼球取血,测定小鼠的白细胞数(WBC)、红细胞数(RBC)、血红蛋白浓度(HGB)、血小板数(PLT)及淋巴细胞百分比(LYM);解剖小鼠,计算肝、脾、肾脏器指数;制作肝、脾、肾、胃组织病理切片,观察脏器病理损伤情况。结果多伞阿魏抗胃癌活性部位对小鼠的最大给药量为5.00 g·kg-1,给药期间多伞阿魏抗胃癌活性部位给药组小鼠给药后出现精神萎靡、倦怠、竖毛、毛发血色暗淡、粪便较粘稠的情况,24 h后均恢复正常,其他一般状况未见异常。此外,在实验14 d内,与空白对照组比较,多伞阿魏抗胃癌活性部位给药组小鼠饮食和体重变化均无明显差异(P>0.05),WBC、RBC、HGB、PLT及LYM均未见统计学差异(P>0.05),小鼠肝、脾、肾脏器指数均未见统计学差异(P>0.05),组织病理结果显示,肝、脾、肾、胃未见明显的病理改变。结论在本实验条件下,多伞阿魏抗胃癌活性部位在14 d内未见严重毒性反应,对小鼠的体重、饮食无明显影响,对小鼠的肝、脾、肾、胃无显著影响,但在给药后2 h内出现一定程度的刺激反应,24 h后均可逐渐恢复正常,这可能是由于多伞阿魏抗胃癌活性部位的神经刺激或胃肠道刺激所导致的应激反应。

Objective To study the acute toxicity and safety of anti-gastric cancer active ingredients of Ferula ferulaeoides so as to provide reference for the research and development of anti-gastric cancer drugs and clinical medications.Methods The mixed suspension of anti-gastric cancer active ingredients of Ferula ferulaeoides was administered intragastrically to mice at the maximum dose.The mice were weighed before and 14 days after administration.The health and death of mice were observed for 14 consecutive days.On the 14th day after administration,serum was collected,and the white blood cell count(WBC),red blood cell count(RBC),hemoglobin concentration(HGB),platelet count(PLT)and lymphocyte percentage(LYM)of mice were determined.The indexes of the liver,spleen and kidney were calculated by dissecting mice.The pathological sections of the liver,spleen,kidney and stomach were prepared to observe the pathological injury to the organs.Results The maximum dosage of anti-gastric cancer active ingredients of Ferula ferulaeoides for mice was 5.00 g·kg-1.During administration,the mice in the group of anti-gastric cancer active ingredients of Ferula ferulaeoides appeared listless,tired,with erection and dark blood color of hair and sticky feces.Compared with blank control group,there was no significant difference in body weight before and 14 d after administration(P>0.05),or in white blood cell count(WBC),red blood cell count(RBC),hemoglobin concentration(HGB),platelet count(PLT)and lymphocyte percentage(LYM)(P>0.05),or in the indexes of the liver,spleen and kidney(P>0.05).Histopathological results showed no significant pathological changes in the liver,spleen,kidney or stomach.Conclusion The anti-gastric cancer active ingredients of Ferula ferulaeoides cause no serious toxic reactions within 14 days,without any significant effect on the weight and diet of mice or on the liver,spleen,kidney and stomach of mice,but irritative reactions may occur within 2h of administration.This may be caused by nerve irritation or gastrointestinal irritation of the anti-gastric cancer active ingredients of Ferula ferulaeoides.