驴血血红蛋白肽的制备工艺研究

Study on the preparation process of donkey blood hemoglobin peptide

为了优化驴血血红蛋白肽的制备工艺,促进驴血产品的深度开发,试验采用分光光度法确定驴血总蛋白分离工艺参数,测定驴血血红蛋白对DPPH和羟基(·OH)自由基的清除能力筛选最佳酶解用酶,并通过响应面法优化驴血血红蛋白酶解工艺。结果表明:4000 r/min离心15 min分离效果最好;4种蛋白酶中以碱性蛋白酶对DPPH和羟基自由基的清除能力最强;驴血血红蛋白肽酶解工艺为酶添加量8260 U/g、pH值9.79、酶解温度55.75℃,此时DPPH自由基清除率达75.99%。

In order to optimize the preparation process of donkey blood hemoglobin peptide and promote the in-depth development of donkey blood products,in this experiment,the process parameters of donkey blood total protein separation were determined by spectrophotometry.The scavenging ability of donkey hemoglobin on DPPH and hydroxyl(·OH)free radicals was determined to screen the best enzymatic hydrolysis enzyme.The enzymatic hydrolysis process of donkey hemoglobin was optimized by the response surface method.The results showed that the separation effect was best when centrifuged at 4000 r/min for 15 min.Among the four proteases,alkaline protease had the strongest average scavenging ability of DPPH and hydroxyl radicals.The enzymatic hydrolysis process of donkey blood hemoglobin peptidation was 8260 U/g,pH 9.79,enzymatic hydrolysis temperature of 55.75℃,and the scavenging rate of DPPH radicals reached 75.99%.