缬草的化学成分研究

Chemical compositions of Valeriana officinalis L.

目的研究败酱科缬草属植物缬草Valeriana officinalis L.的化学成分。方法采用硅胶柱色谱、MCI柱色谱、ODS柱色谱、Sephadex LH-20凝胶柱色谱和SP-HPLC等方法对缬草80%乙醇提取物正丁醇部位与水部位进行分离纯化,根据理化性质和NMR、MS等波谱技术鉴定化合物结构。结果从缬草中分离得到20个化合物,分别鉴定为valerol A(1)、pinoresinol-4-O-α-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside(2)、junipetrioloside A(3)、coniferin(4)、eugenylβ-rutinoside(5)、xiecaoside E(6)、benzyl primeveroside(7)、3-caffeoylquinic acid methyl ester(8)、对羟基苯甲醛(9)、vanillic acid(10)、吲哚-3-甲醛(11)、thymine DNA nucleotides(12)、蔷薇酸(13)、熊果酸(14)、δ-hederin(15)、蔗糖(16)、正十四烷酸甲酯(17)、1-t-butoxyoctane(18)、硬脂酸(19)、2,5-二甲基-3-己酮(20)。结论化合物3为从败酱科植物中首次分离得到,化合物2、4、7、12、16为从缬草属植物中首次分离得到,化合物6、8、15为从缬草中首次分离得到。

Objective To determine the chemical compositions of Valeriana officinalis L.Methods The n-butanol part and water part of the 80%ethanol extract of Valeriana officinalis L.was systematically separated by silica gel column chromatography,MCI column chromatography,ODS column chromatography,Sephadex LH-20 gel column chromatography and SP-HPLC.The structures were identified by NMR,MS and other spectra analysis combined with physical and chemical properties.Results The structures of 20 compounds were identified as valerol A(1),pinoresinol-4-O-α-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside(2),junipetrioloside A(3),coniferin(4),eugenylβ-rutinoside(5),xiecaoside E(6),benzyl primeveroside(7),3-caffeoylquinic acid methyl ester(8),p-hydroxybenzaldehyde(9),vanillic acid(10),indole-3-carboxaldehyde(11),thymine DNA nucleotides(12),rosolic acid(13),ursolic acid(14),δ-hederin(15),sucrose(16),N-tetradecanoic acid methyl ester(17),1-t-butoxyoctane(18),stearic acid(19),and 2,5-dimethyl-3-hexanone(20).Conclusion Compound 3 has been first isolated from Valerianaceae,compounds 2,4,7,12 and 16 have been first isolated from genus Valeriana,and compounds 6,8 and 15 have been first isolated from this plant.