砂仁渗漉汁脱色工艺优化及对肠道损伤的保护作用

Optimization of decolorization process for Amomum villosum percolating juice and its protective effect on intestinal injury

为了降低砂仁渗漉汁的色度,采用D941阴离子交换树脂对砂仁渗漉汁进行脱色,并初步探索砂仁渗漉汁的药理作用。以脱色率为评价指标,通过单因素试验和响应面试验优化砂仁渗漉汁的脱色工艺条件,并建立脾虚泄泻大鼠模型,观察并测定试验前后各组别大鼠的胃动素、胃泌素水平以及结肠病例切片情况。结果表明,最佳脱色工艺条件为砂仁渗漉汁与树脂质量比2∶1,脱色pH 6.9,脱色时间6 h,脱色温度51℃。在此最佳脱色工艺条件下,砂仁渗漉汁脱色率为(68.96±1.05)%,乙酸龙脑酯保留率为(79.76±0.95)%,以基酒作为溶剂制备的砂仁渗漉汁能有效降低基酒对脾虚大鼠的胃肠道损伤,保护肠道屏障。

In order to reduce the chromaticity of Amomum villosum percolating juice,the A.villosum percolating juice was decolorized by D941 anion exchange resin,and the pharmacological effect of A.villosum percolating juice was preliminarily explored.With the decolorization rate as the evaluation index,the decolorization conditions of A.villosum percolation juice were optimized by single factor tests and response surface tests.The rat model of splenic deficiency and diarrhea was established,and the levels of motilin and gastrin in each group of rats and the sections of colon cases were observed and measured before and after the experiment.The results showed that the optimum decolorization conditions were as follows:mass ratio of A.villosum percolation juice to resin 2∶1,decolorization pH 6.9,time 6 h,and temperature 51℃.Under the optimum decolorization conditions,the decolorization rate of A.villosum percolation juice was(68.96±1.05)%,and the retention rate of bornyl acetate was(79.76±0.95)%.The percolation juice of A.villosum prepared with base liquor as solvent could effectively reduce the damage of base liquor to gastrointestinal tract of spleen deficiency rats and protect intestinal barrier.