恶性黑色素瘤组织中circRNA_0020710和circRNA_0079593的水平表达及其临床价值

Expression and Clinical Value of circRNA_0020710 and circrNA_0079593 in Malignant Melanoma Tissues

目的 探讨环状核糖核酸(circular RNA, circRNA)_0020710及环状核糖核酸(circular RNA, circRNA)_0079593在恶性黑色素瘤组织中的表达及其临床价值。方法 选取2019年1月~2022年3月保定市第二中心医院收治的61例恶性黑色素瘤患者,采用实时荧光定量PCR法检测恶性黑色素瘤组织及正常组织circ RNA_0020710及circ RNA_0079593表达水平,并分析circ RNA_0020710及circ RNA_0079593表达水平与恶性黑色素瘤患者临床特征的关系。应用受试者工作特征(ROC)曲线分析circ RNA_0020710及circ RNA_0079593对恶性黑色素瘤诊断及其预测淋巴结转移的价值。结果 与正常组织相比,恶性黑色素瘤组织circ RNA_0020710(4.95±2.37 vs 1.84±0.92)及circ RNA_0079593(2.70±1.15 vs 0.91±0.34)表达水平均明显升高,差异具有统计学意义(t=18.946,15.248,均P<0.001)。恶性黑色素瘤患者circ RNA_0020710及circ RNA_0079593表达水平升高与组织分级Ⅲ~Ⅳ级、肿瘤部位、低分化及淋巴结转移有关(t=9.620~20.713,均P<0.001)。ROC曲线显示,circ RNA_0020710及circ RNA_0079593诊断恶性黑色素瘤及预测淋巴结转移的曲线下面积分别为0.847(95%CI:0.787~0.905),0.806(95%CI:0.748~0.864),0.887(95%CI:0.829~0.948)和0.842(95%CI:0.793~0.907),其敏感度和特异度均较好。结论 circ RNA_0020710及circ RNA_0079593水平在恶性黑色素瘤组织中明显升高,与患者临床特征有关,可作为该病诊断及病情判断的分子标志物。

Objective To investigate the expression and clinical value of circular RNA(circRNA)_0020710 and circular RNA(circRNA)_0079593 in malignant melanoma tissues.Methods 61 patients with malignant melanoma admitted to the Second Central Hospital of Baoding from January 2019 to March 2022 were selected.The levels of circRNA_0020710 and circrNA_0079593 in malignant melanoma tissues and normal tissues were detected by real-time PCR.Analyze the relationship between the expression of circRNA_0020710 and circRNA_0079593 levels and the clinical characteristics of malignant melanoma patients.The ROC curve was used to analyze the value of circRNA_0020710 and circRNA_0079593 in the diagnosis and prediction of lymph node metastasis of malignant melanoma.Results The levels of circRNA_0020710(4.95±2.37 vs 1.84±0.92)and circRNA_0079593(2.70±1.15 vs 0.91±0.34)in malignant melanoma tissues were significantly higher than those in normal tissues(t=18.946,15.248,all P<0.001).The increased expression levels of circRNA_0020710 and circRNA_0079593 in malignant melanoma patients were associated with tissue gradeⅢ~Ⅳ,tumor location,low differentiation and lymph node metastasis(t=9.620~20.713,all P<0.001).ROC curve showed that the areas under the curve of circRNA_0020710 and circRNA_0079593 for diagnosing malignant melanoma and predicting lymph node metastasis were 0.847(95%CI:0.787~0.905),0.806(95%CI:0.748~0.864),0.887(95%CI:0.829~0.948)and 0.842(95%CI:0.793~0.907),respectively,with good sensitivity and specificity.Conclusion The levels of circRNA_0020710 and circRNA_0079593 were significantly increased in malignant melanoma tissues,which were related to the clinical characteristics of patients.It can be used as molecular markers for the diagnosis and diagnosis of the disease.