糖尿病阴证大鼠创面模型的制备

Establishment of Wound Model of Diabetes Rats with Yin Syndrome

目的:探讨糖尿病阴证大鼠创面模型的制备方法。方法:雄性SD大鼠36只,随机分为正常创面组、糖尿病创面组和糖尿病阴证创面组。除正常创面组外,其余两组大鼠采用2 mL高脂乳剂灌胃14 d,予以一次性腹腔注射50 mg/kg STZ-柠檬酸钠混合液,糖尿病阴证创面组大鼠按2 mg/(kg·d)肌内注射氢化可的松;同时正常创面组灌胃饮用水,腹腔注射生理盐水。7 d后3组大鼠均进行创面手术,观察大鼠一般情况,进行OGTT试验,观察胰腺组织形态以及创面愈合率。结果:糖尿病阴证创面组造模成功率91.7%;与正常创面组比较,糖尿病创面组与糖尿病阴证创面组大鼠血糖明显升高(P<0.05),且存在胰岛素抵抗;脏器指数显示,糖尿病阴证创面组较其余两组降低(P<0.05);胰腺HE染色表明,正常创面组大鼠胰腺组织肥厚,胰岛完整,而糖尿病创面组与糖尿病阴证创面组胰岛边界模糊,β细胞消融。创面形态方面,糖尿病阴证创面组相较于正常创面组和糖尿病创面组的愈合率较低(P<0.01),HE染色可见糖尿病阴证创面组全层皮肤较前两组薄,表皮生长不平整,炎细胞浸润,细胞排列紊乱。结论:给予SD大鼠2 mL高脂乳剂灌胃14 d后,予以一次性腹腔注射50 mg/kg STZ-柠檬酸钠混合液联合2 mL/kg肌内注射氢化可的松注射液7 d后行创面手术,可建立稳定高效的糖尿病阴证大鼠创面模型。

Objective:To explore the preparation method of wound model of diabetes rats with yin syndrome.Methods:36 Sprague Dawley male rats were randomly divided into three groups:normal wound group(group A),group of diabetes and wound(group B),and group of diabetes and wound with yin syndrome(group C).2 mL high-fat emulsion was administered by gavage for 14 days,50 mg/kg STZ sodium citrate mixture was intraperitoneally injected once,and 2 mg/kg/d hydrocortisone was given by intramuscular injection.After 7 days,wound was made surgically.General condition of the animals,OGTT test,pancreatic tissue morphology,and wound healing rate were recorded.Results:The success rate of modeling for group C was 91.7%.Compared with group A,the blood glucose of group B and group C was significantly increased(P<0.05),and there was insulin resistance in these 2 groups.Group C gave lower organ index than the other two groups(P<0.05).HE staining of pancreatic tissue showed that the pancreatic tissue of rats in the group A was hypertrophic and the islets were intact,while the boundary of the islets in the group B and group C was blurred and the β cells were ablated.The wound healing rate was lower in group C than that in group A and group B(P<0.01),and HE staining showed that the full-layer skin at the wound in group C was thinner than that in the group A and B.The epidermal growth was uneven,there were inflammatory cells infiltrated,and the cell arrangement was in disorder in group C.Conclusion:Based on this,we can establish a stable wound model of diabetes rats with yin syndrome.