急性冠脉综合征气虚血瘀患者LC-MS血清代谢组学分析

LC-MS Serum Metabonomic Analysis in Patients with Acute Coronary Syndrome with Qi Deficiency and Blood Stasis

目的:研究急性冠脉综合征气虚血瘀患者的血清代谢组学变化情况,找出其相关的生物标志物。方法:检测40例急性冠脉综合征气虚血瘀患者及40例健康人的血清标本,采用液相色谱-质谱联用(LC-MS)非靶向法进行代谢组学分析其异常代谢物及代谢产物。结果:共纳入观察组40例、对照组40例样本进行检测,发现急性冠脉综合征气虚血瘀患者与健康人的显著差异代谢物32种,关键代谢通路共4条;蕨内酰胺、烟酰胺、次黄嘌呤、乙酰肼、7-甲基鸟嘌呤、苯丙氨酸、三丙酸甘油酯、左旋缬氨酸、左旋正亮氨酸、谷氨酸、乙醇酸、花生四烯酸、α-D-葡萄糖及肌酐等代谢物可作为鉴别二者的潜在生物标志物;涉及的关键通路包括精氨酸和脯氨酸代谢,嘧啶代谢,甘氨酸、丝氨酸和苏氨酸代谢,谷胱甘肽代谢等。结论:急性冠脉综合征气虚血瘀患者与健康人血清代谢组分存在显著差异,代谢组学相关研究对探求其生物学本质以及中医“证”的实质具有一定指导意义。

Objective:We aimed to investigate the metabolomic changes and find associated biomarkers in serum of the patients with qi deficiency and blood stasis in acute coronary syndrome.Methods:Serum specimens were collected for 40 patients with qi deficiency and blood stasis and with acute coronary syndrome and for 40 healthy adults were collected in this study.Metabolomics analysis of abnormal metabolites and metabolites was performed using liquid chromatography-mass spectrometry(LC-MS)non targeted method.Results:We found that 32 metabolites were significantly different between the two groups,and 4 key metabolic pathways had been identified.Metabolites,including pterinactam,nicotinamide,hypoxanthine,acetylhydrazine,7-methylguanine,phenylalanine,tripropionate,L-valine,L-norleucine,glutamic acid,glycolic acid,arachidonic acid,α-D-glucose,and creatinine,can serve as potential biomarkers for differential diagnosis of the two groups.The key pathways involved include arginine and proline metabolism,pyrimidine metabolism,glycine,serine and threonine metabolism,glutathione metabolism,etc.Conclusion:There is a significant difference in serum metabolic components between patients with acute coronary syndrome with qi deficiency and blood stasis and healthy individuals.Metabolomics related research has certain guiding significance in exploring its biological essence and the essence of traditional Chinese medicine"syndrome".