SERS免疫层析技术在快速检测领域的研究进展

Research progress of surface-enhanced Raman scattering(SERS)-based immunochromatography in rapid detection

免疫层析技术具有快速、成本低廉、操作简便、可视化分析等优势,已广泛应用于食品安全、环境监测及临床诊断等领域。基于颜色深浅进行结果判读的传统免疫层析技术,存在检测灵敏度低、难以实现定量分析等问题。表面增强拉曼散射(SERS)与免疫层析联合的技术提供了一个强大的分析平台,能够实现对分析物的多重、超灵敏及定量检测。本文从检测原理及类型、标签筛选、分析策略设计等方面简述了SERS免疫层析快速检测技术,归纳了其在致病菌、病毒、农兽药残留、真菌毒素等危害物检测方面的最新研究进展,并且对SERS免疫层析技术的研究方向与发展前景进行了分析和展望。

Immunochromatography has been widely used in food safety,environmental monitoring and clinical diagnosis due to its advantages of fast,low cost,simple operation and visual analysis.Traditional immunochromatography,which interprets results based on color depth,has many challenges,such as low detection sensitivity and difficulty in quantitative analysis.SERS combined with immunochromatography provides a powerful analytical platform,which can realize multiple,ultra-sensitive and quantitative detection of analytes.This paper briefly describes the rapid detection technology of SERS immunochromatography from the aspects of detection principles and types,label screening,and analysis strategy design,summarizes its latest research progress in the detection of hazards such as pathogenic bacteria,viruses,agricultural,veterinary drug residues and mycotoxins,and also prospects the research direction and further development of SERS immunochromatographic technology.