防己结构中红外光谱研究

Study on the middle infrared spectroscopy of the radix stephaniae tetrandrae structure

采用MIR光谱(包括一维MIR光谱、二阶导数MIR光谱、四阶导数MIR光谱及去卷积MIR光谱)开展了防己结构的研究。试验发现,防己结构的红外吸收模式主要包括ν_(asCH_(3-防己))、ν_(asCH_(2-防己))、ν_(sCH_(3-防己))、ν_(sCH_(2-防己))、ν_(C=O_(-防己))、ν_(C=C_(-防己))、δ_(CH_(2-防己))、δ_(asCH_(3-防己))、δ_(sCH_(3-防己))和ν_(C-O_(-防己))。研究发现,防己结构的去卷积MIR光谱的谱图分辨能力要优于相应的一维MIR光谱、二阶导数MIR光谱和四阶导数MIR光谱。本项研究拓展了MIR光谱技术在重要的传统中药(防己)结构的研究范围。

The radix stephaniae tetrandrae structure was studied by MIR spectroscopy(including one-dimensional MIR spectroscopy,second-order derivative MIR spectroscopy,fourth-order derivative MIR spectroscopy and deconvolution MIR spectroscopy).The experiment found that the infrared absorption modes of radix stephaniae tetrandrae structure mainly included:ν_(asCH3-radix stephaniae tetrandrae),ν_(asCH2-radix stephaniae tetrandrae),ν_(sCH3-radix stephaniae tetrandrae),ν_(sCH2-radix stephaniae tetrandrae),ν_(C=O-radix stephaniae tetrandrae),ν_(C=C-radix stephaniae tetrandrae),δ_(CH2-radix stephaniae tetrandrae),δ_(asCH3-radix stephaniae tetrandrae),δ_(sCH3-radix stephaniae tetrandrae) andν_(C-O-radix stephaniae tetrandrae).It was found that the deconvolution MIR spectra of radix stephaniae tetrandrae molecules had better spectral resolution than the corresponding one-dimensional MIR spectra,second-order derivative MIR spectra and fourth-order derivative MIR spectra.This study expanded the research scope of MIR spectroscopy in the molecular structure of important traditional chinese medicine(radix stephaniae tetrandrae).